Organ reengineering through development of a transplantable.pdfVIP

T e c h n i c a l R e p o R T s Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix 1 1,6 1,6 1 1,2 Basak E Uygun , Alejandro Soto-Gutierrez , Hiroshi Yagi , Maria-Louisa Izamis , Maria A Guzzardi , 1 1 3 1 1,4 5 Carley Shulman , Jack Milwid , Naoya Kobayashi , Arno Tilles , Francois Berthiaume , Martin Hertl , Yaakov Nahmias1,6, Martin L Yarmush1,4 Korkut Uygun 1 . d e v r e Orthotopic liver transplantation is the only available treatment Tissue engineering approaches have thus far been able to partially s e for severe liver failure, but it is currently limited by organ improve cell engraftment in animal models by enhancing cell­to­cell r s shortage. One technical challenge that has thus far limited the contact and providing nonimmunogenic matrices before transplan­ t h development of a tissue-engineered liver graft is oxygen and tation 15. However, these approaches have been limited by oxygen g i r l nutrient transport. Here we demonstrate a novel approach to and nutrient diffusion to thin, two­dimensional structures that are l A generate transplantable liver grafts using decellularized liver transplanted into highly vascularized regions 16–18. Practical consid­ . c matrix. The decellular