文献1的.【nc-2013-04-22】trapping red blood cells in living animals using optical tweezers.pdf

ARTICLE Received 31 Jul 2012 | Accepted 22 Mar 2013 | Published 23 Apr 2013 DOI: 10.1038/ncomms2786 Trapping red blood cells in living animals using optical tweezers 1,* 1,2,* 1 1 1 Min-Cheng Zhong , Xun-Bin Wei , Jin-Hua Zhou , Zi-Qiang Wang Yin-Mei Li The recent development of non-invasive imaging techniques has enabled the visualization of molecular events underlying cellular processes in live cells. Although microscopic objects can be readily manipulated at the cellular level, additional physiological insight is likely to be gained by manipulation of cells in vivo, which has not been achieved so far. Here we use infrared optical tweezers to trap and manipulate red blood cells within subdermal capillaries in living mice. We realize a non-contact micro-operation that results in the clearing of a blocked microvessel. Furthermore, we estimate the optical trap stiffness in the capillary. Our work expands the application of optical tweezers to the study of live cell dynamics in animals. 1 Department of Optics and Optical Engineering and Hefei National Laboratory for Physical Science at the Microscale, University of Science and Technology of China, Hefei 230026, China. 2 Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China. * These authors contributed equally to this work. Correspondence and requests for materials should be addressed to Y.-M.L. (email: liyinmei@) or to X.-B.W. (email: xwei01@). NATURE COMMUNICATIONS | 4:1768 | DOI: 10.1038/ncomms2786 | /naturecommunications 1 2013 Macmillan Publishers Limited. All rights reserved. ARTICLE