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Journal of Experimental Botany, Vol. 57, No. 4, pp. 827–835, 2006 doi:10.1093/jxb/erj066 Advance Access publication 31 January, 2006 This paper is available online free of all access charges (see /open_access.html for further details) RESEARCH PAPER A proteomic approach to analysing responses of Arabidopsis thaliana callus cells to clinostat rotation 1 1, 2 2 2 Hui Wang , Hui Qiong Zheng *, Wei Sha , Rong Zeng and Qi Chang Xia 1 Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China 2 Research Center for Proteome Analysis, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200032, China Received 2 August 2005; Accepted 18 November 2005 Abstract that clinostat rotation of Arabidopsis callus cells has a significant impact on the expression of proteins Callus cells of Arabidopsis thaliana (cv. Landsberg involved in general stress responses, metabolic path- erecta) were exposed for 8 h to a horizontal clinostat ways, gene activation/transcription, protein synthesis, rotation (H, simulated weightlessness), a vertical clino- and cell wall biosynthesis. stat rotation (V, clinostat control), or a stationary con- trol (S) growth condition. The amount of glucose and Key words: Arabidopsis thaliana, cal