a novel third generation uric acid biosensor using uricase electro-activated with ferrocene on a nafion coated glassy carbon electrode：一种新型第三代尿酸生物传感器采用尿酸酶电在nafion修饰玻碳电极的二茂铁活性.pdfVIP

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a novel third generation uric acid biosensor using uricase electro-activated with ferrocene on a nafion coated glassy carbon electrode：一种新型第三代尿酸生物传感器采用尿酸酶电在nafion修饰玻碳电极的二茂铁活性.pdf

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a novel third generation uric acid biosensor using uricase electro-activated with ferrocene on a nafion coated glassy carbon electrode：一种新型第三代尿酸生物传感器采用尿酸酶电在nafion修饰玻碳电极的二茂铁活性

Bioelectrochemistry 102 (2015) 1–9 Contents lists available at ScienceDirect Bioelectrochemistry journal home page: /locate/bioel eche m A novel third generation uric acid biosensor using uricase electro-activated with ferrocene on a Naﬁon coated glassy carbon electrode Tanushree Ghosh a, Priyabrata Sarkar a,⁎, Anthony P.F. Turner b a Biosensor Laboratory, Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C. Road, Kolkata 700 009, India b Biosensors and Bioelectronics Centre, IFM, Linköping University, S-58183 Linköping, Sweden a r t i c l e i n f o a b s t r a c t Article history: A new uric acid biosensor was constructed using ferrocene (Fc) induced electro-activated uricase (UOx) depos- Received 26 May 2014 ited within Naﬁon (Naf) on glassy carbon electrode (GCE). Electro-activation of UOx was successfully achieved by Received in revised form 5 November 2014 cyclic voltammetry through the electrostatic interaction of Fc with Trp residues within the hydrophobic pockets Accepted 8 November 2014 in UOx. The Naf/UOx/Fc composite was characterised by AFM, FTIR and EDX to ensure proper immobilisation. The Available online 11 November 2014 interaction of Fc with the enzyme was analysed by Trp ﬂuorescence spectroscopy and the α-helicity of the pro- Keywords: tein was measured by CD spectropolarimetry. The charge transfer resistance (Rct), calculated from electrochem- Uricase