原创力文档a portable, shock-proof, surface-heated droplet pcr system for escherichia coli detection：一种便携式、防震、表面加热的液滴的大肠杆菌检测系统.pdfVIP
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a portable, shock-proof, surface-heated droplet pcr system for escherichia coli detection：一种便携式、防震、表面加热的液滴的大肠杆菌检测系统
Biosensors and Bioelectronics 74 (2015) 360–368
Contents lists available at ScienceDirect
Biosensors and Bioelectronics
journal homepage: /locate/bios
A portable, shock-proof, surface-heated droplet PCR system for
Escherichia coli detection
Scott V. Angus a,1, Soohee Cho a,1, Dustin K. Harshman b, Jae-Young Song c,
Jeong-Yeol Yoon a,b,n
a Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, AZ 85721, USA
b Biomedical Engineering Graduate Interdisciplinary Program, The University of Arizona, Tucson, AZ 85721, USA
c Viral Disease Division, Animal and Plant Quarantine Agency, Anyang-si, Gyeonggi-do 430-757, Republic of Korea
a r t i c l e i n f o a b s t r a c t
Article history: A novel polymerase chain reaction (PCR) device was developed that uses wire-guided droplet manip-
Received 12 May 2015 ulation (WDM) to guide a droplet over three different heating chambers. After PCR amplification, end-
Accepted 12 June 2015 point detection is achieved using a smartphone-based fluorescence microscope. The device was tested
Available online 29 June 2015
for identification of the 16S rRNA gene V3 hypervariable region from Escherichia coli genomic DNA. The
Keywords: lower limit of detection was 103 genome copies per sample. The device is portable with smartphone-
16S rRNA based end-point detection and provides the assay results quickly (15 min for a 30-cycle amplification)
Conta
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