a real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus：黄热病病毒快速检测的实时逆转录环介导等温扩增法.pdf

Journal of Virological Methods 193 (2013) 23–27 Contents lists available at SciVerse ScienceDirect Journal of Virological Methods j o u r n a l h o m e p a g e: w w w. elsev /locate/jviromet Short communication A real-time reverse transcription loop-mediated isothermal amplification assay for the rapid detection of yellow fever virus Allan ole Kwallah a,d , Shingo Inoue b,∗ , Anne W.T. Muigai a , Toru Kubo b , Rosemary Sang c , Kouichi Morita b , Matilu Mwau d a Institute of Tropical Medicine and Infectious Diseases (ITROMID),Jomo Kenyatta University of Agriculture and Technology (JKUAT),Juja, 62000-00200, Kenya b Department of Virology, Institute of Tropical Medicine, Nagasaki University and GCOE Program, 1-12-4 Sakamoto, Nagasaki, 852-8523,Japan c Centrefor Virus Research (CVR), Kenya Medical Research Institute (KEMRI), Nairobi, 54840-00200, Kenya d Centrefor Infectious and Parasitic Diseases Control Research (CIPDCR), Kenya Medical Research Institute (KEMRI), Busia, 3-50400, Kenya a b s t r a c t Article history: Yellow fever, a mosquito-borne disease, is an important viral hemorrhagic fever in Africa and South Received 21 August 2012 America where it is endemic. Detection of yellow fever virus (YFV) in Africa remains a challenge due Received in revised form 2 May 2013 to a lack of highly specific tests. The aim of this study was to develop and optimize a rapid detection Accepted 13 May 2013 reverse transcription loop-mediated isothermal amplification (RT-LAMP) for YFV. The RT-LAMP was done Available online 18 May 2013 ◦