稳定转染靶向bl-2的shrna对胃癌sgc-7901细胞株5-fuddp敏感性的影响.pdf

Effects of shRNA tobcl·2inSGC-7901 stablyexpressingtargeted cells chemosensitivity ective：To theeffectofstable shRNA to obj investigate expressiontargeted bcl．2inSGC．7901 cells． Methods：AshRNA vectorthat the small expressed expressesspecific RNA bcl一2mRNAwasconstructedandtransfectedinto hairpintargeting SGC-7901cells．The shRNAcellswereselected G418 stablyexpressing by and cultured inhalftheantibiotic with continuously 5-FuorDDP．Thebcl．2mRNAwasobservedwith withMIT and proliferationcapacity assay the statewithflow apoptotic cytometry． Results：InSGC-7901cells shRNA,theof stablyexpressing expression bcl-2 mRNAweredecreased，butcellular and proliferationcapacity ratewere of5-Fuwas ofDDP apoptotic normal．IC50 14．36±1．63mg／L，If50 was ratewasincreased． 2．53±O．46mg／L，apoptosis Conclusion：ThedemonstratedthatshRNAhas effectto study long-term decreasethebcl-2mRNA andenhancethe transcription butcellular and werenormal．which rate proliferationcapacityapoptotic that bcl一2 RNAibea in impliesregulategenesby may potentialapproach cancer