急性白血病gs-π与lrp基因及编码蛋白的临床研究与检测.pdf

中 文 摘 要 leukemi- 目的多药耐药性 multi—drugresistance，MDR 是导致急性白血病 Acute resistance—related 和肺耐药相关蛋白 1ung protein，LRP 基因及其编码蛋白的检测，探 讨两种基因及其编码蛋白与急性白血病患者多药耐药性的关系，它们之间是否存在相关 性，以及与AL临床分型、白细胞计数的关系。方法采用实时荧光定量PCR RQ— 达量在不同外周血白细胞计数组、不同临床分型组 急性淋巴细胞白血病√虹L、急性非淋 巴细胞白血病ANLL 之间的差异均不显著。初治组与完全缓解组，难治组与完全缓解 表达均有极显著性差异 P 0．01 o结论外周血白细胞计数与白血病分型可能不影 临床耐药密切相关oGST一iT和LRP基因通过不同机制引起多药耐药，二者联合检测较 单独测定某一基因对白血病评定预后可能更具临床意义。应用实时荧光定量RQ—PCR 检测基因与免疫组化检测蛋白均可提示预后。 关键词LRPC,ST一面MDR白血病 andexamination The of investigation GST—qf、 LRP andtheirrelated inacuteleukemia gene proteins Abstract failture can resultinthe ofa· resistance ObjectiveMultidrng eventually chemotherapy cute examinationof and resistance—related leukemia．ThroughGST一面genelung protein telated betweenthemandthe andtheir show：the U心 gene proteins，80舾torelationship resistanceofacuteleukemia correlationbetweenGST一仃and multi—drug patient；the LRP； of between the betweenthemandclinical acute relationship type leukemia；therelationship Realtimefluorescent reverse themandWBCcount．Methods quantiWlvetranscriptionpoly- used andLRP in me瑚echain todetermineGST一1r peripher- reaction RQ-PCR W88 gene of44AL and27normal immuno—his- albloodmononuclearcell subjects．AndS—P patients LRP in40AL tochemicalmethodwasusedtoexaminateGST一订and patients staining protein and20normal correlation testwereused．Results subjects．ANOV，ttest，line analysis，X2 LRP had110 differenceindifferent ofGST一1ror WBC white— Expression gene significant anddifferentclinical leukemia， blood—cell countgroup typegroup acutelymphohlastic of Was expression ALL；acutenonlymphoblasficleukemia，AmJ． ．ComparisionGsr一霄gene differentbetween and remission and significant primary@xmpcomplete group，refractorygroup remission ofLRP in expressionprimarygroup complete group P 0．01 ．Comparisiongene and remissionand showed differ- refractory significant refractorygroup，completegroup group showedthereWasnocorrelationbetweenthe ofGST--IT ence P 0．01 ．Statistics expression re． andIXP inthe differen