四川小麦地方品种AS1643中低分子量谷蛋白基因的克隆及蛋白质的二级结构预测.pdfVIP

HEREDITAS (Beijing) 2007 7 , 29( 7): 858865 ISSN 0253-9772 DOI: 10.1360/yc-007-0858 AS1643 1,2,3, 1, 2,3 , 2,3 1. , 625014; 2. , 625014; 3. , 611830 : P1/P2, PCR AS1643 DNA , 1 900 bp , pMD18-T , , 1 LMW-AS1643 (GenBank : EF190322), 909 bp, 302 , LMW-AS1643 , LMW-GS , 93.40% , LMW-AS1643 , , 67.90 %, α-, 30.46 %, β- , 1.64 % : ; ; ; Cloning of low-molecular-weight glutenin subunit gene from Sichuan wheat landrace AS1643 and its secondary structure prediction 1,2,3 1 2,3 2,3 CHEN Hua-Ping , HUANG Qian-Ming , WEI Yu-Ming , ZHENG You-Liang 1. College of Biology and Science, Sichuan Agricultural University, Ya,an 625014, China; 2. Ministry of Education for Crop Genetic Resources and Improvement in Southwest China , Sichuan Agricultural University, Ya,an 625014, China; 3. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830 China Abstract: The full-length coding region (open reading frame, ORF) of low-molecular-weight glutenin subunit (LMW-GS ) gene was amplified from Sichuan wheat landrace accession AS1643 by using the primer pairs P1/P2, which were designed according to wheat LMW-GS conservative domains. The amplified DNA fragment was separated and recovered from aga- rose gel, subsequently cloned into pMD18-T vector, and then transformed into E. coli strain DH5α. One positive c