大肠杆菌TorS_TorR二组分体应答蛋白TorR对DNA复制起始的影响.pdfVIP

Hereditas (Beijing) 2015 年3 月, 37(3): 302 ―308 研究报告 大肠杆菌TorS/TorR 二组分体应答蛋白TorR 对DNA 复制起始的影响 姚远，乔佳鑫，李静，李慧，莫日根 内蒙古大学生命科学学院，呼和浩特 010021 摘要:二组分体作为一种信号转导系统在细菌中普遍存在，能够感知外界环境变化并做出应答。细菌中 CckA/CtrA、ArcA/ArcB 和PhoP/PhoQ 二组分体与DNA 复制起始和细胞分裂相关，但目前还未见TorS/TorR 二 组分体对细胞周期及 DNA 复制影响的相关报道。大肠杆菌 TorS/TorR 二组分体能够监测细胞周围氧化三甲胺 (Trimethylamine oxide, TMAO) 的浓度变化，但其是否影响DNA 复制起始呢？文章利用流式细胞仪检测了ΔtorS 和 ΔtorR 突变体菌株的复制式样。结果发现，ΔtorS 突变菌株每个细胞复制起始原点数目和倍增时间与野生型 细胞一致，而ΔtorR 突变菌株每个细胞复制起始原点数目多于野生型细胞，说明复制起始发生时间比野生型细 胞早。但是过表达TorR 蛋白或者共同表达 TorS 和TorR 蛋白都不能使ΔtorR 突变体表型恢复为野生型表型。 而在野生型和ΔtorR 突变细胞中过表达SufD 蛋白能使复制起始提早发生，在ΔtorR 和ΔsufD 双突变细胞中复 制起始延迟。所以，TorR 可能通过改变sufD 基因的表达来间接影响染色体复制起始。 关键词: TorS/TorR 二组分体；应答蛋白TorR；DNA 复制；大肠杆菌 The effects of TorR protein on initiation of DNA replication in Escherichia coli Yuan Yao, Jiaxin Qiao, Jing Li, Hui Li, Morigen School of Life Sciences, Inner Mongolia University, Hohhot 010021, China Abstract: The two-component systems, which could sense and respond to environmental changes, widely exist in bacteria as a signal transduction pathway. The bacterial CckA/CtrA, ArcA/ArcB and PhoP/PhoQ two-component systems are associated with initiation of DNA replication and cell division, however, the effects of the TorS/TorR system on cell cycle and DNA replication remains unknown . The TorS/TorR system in Escherichia coli can sense changes in trimethylamine oxide (TMAO) concentration around the cells. However, it is unknown if it also affects initiation of DNA replication. We detected DNA replication patterns in ΔtorS and ΔtorR mutant strains by flow cytometry. We found that the average number of replication origins (oriCs) per cell and doubling time in ΔtorS mutants were the same while the average number of oriCs in ΔtorR mutants was increased compared