cox-2在不肿瘤中组织原位表达谱的研究.pdfVIP

浙江夫学硕一学位论文 摘要 目的用组织芯片技术检NCOX．2在食管癌、胃癌、结肠癌、原发性肝癌、 乳腺癌、肾癌、肺癌、甲状腺癌和膀胱癌9种常见肿瘤中的表达。 方法应用ZM一1型组织芯片制备系统制作9种肿瘤的组织芯片，通过 理类型、分化程度、浸润程度、淋巴结转移的关系。． 组织芯片蜡块，COx．2在食管癌、胃癌、结肠癌、原发性肝癌、乳腺癌、肾癌、 两组之间，COX．2的阳性表达差异无显著性(P0．005)。 结论COX．2在9种肿瘤组织中普遍高表达，与淋巴结转移、浸润无明显 相关，可以作为临床选择治疗方案和对肿瘤进展及预后判断的参考指标之一。 关键词： 环氧化酶组织芯片免疫组化zM一1 塑垩查兰竺!兰竺兰兰 。。 Abstract Todetectethe of 0bjecfive protein expression tissane intheninehumanfrefent included microarray carcinoms，whichesophagus, carcinoma，colorectalcarcinoma,liver gastric carcinoma,breastcarcinoma,kidney carcinomaandbladdercarcinoma． carcinoma,lungcarcinoma,thyroid MethodsAtissue ofnine humancarcinomaWas microarrayfrequent analyzed anti—COX··2monoclonal astatisticalalia- immunohistochemically,usingantibody,and Was toanswerthe with differentiated lysis employed relationship degree，infiltrative nodemetastasis． depth，lymph ResultsZM-1tissue block tissue By microarrayparaffinmaker，aparaffin blockwith106tissue included carcinoma，colo- sample，whichesophagus，gastric rectal carci· carcinoma,livercarcinoma,breast carcinoma,kidneycarcinoma，lung carcinomaandbladdercarcinoma．TheofCOX一2inthese noma,thyroid positives tumorswere 60．00％and ofCOX-2iscorrelatedwith 41．67％respectively,theexpression