satb1特异rnai慢病毒载体构建及其对乳腺癌细胞增殖影响.pdf

SATBl特异的RNAi慢病毒载体构建及其对 乳腺癌细胞增殖影响 摘要： 慢病毒表达载体，观察抑制其基因表达后对高侵袭性人乳腺癌细胞株MDA．MB．231 增殖的影响。 表达情况； 胞增殖的影响。 碱基序列及插入位点完全正确；4．构建的RNAi慢病毒感染人乳腺癌细胞MDA-MB 扰组和对照组相比，细胞增殖率明显下降。 2．SATBl表达被抑制后对高侵袭性的人乳腺癌细胞株的增殖有显著抑制作用。由此 可推出SATBl可能在乳腺癌浸润转移中扮演一种重要的作用。 关键词：SATBlRNAi慢病毒乳腺癌转移 4 ConstructionofalentiviralvectorforRNAinterferenceofSATBI gene andits effecton silencing proliferation inbreast cancercells． Abstract Purpose： Theaimwasto the of rich SATB A-T exploreexpression1(theSpecial sequencebinding breastcancercells．Alentiviral vectorforRNA protein)in expression ofSATB1 was its effectin human constructed，then gene genesilencing highlyaggressive breast cell cancerlineMDA．MB．231 wasassessed． Methods： 1．The ofSATB1 mRNAinbreastcancer observed expression cells(MDA—MB一231)was by Real-timefluorescent PeR．2．The ofSATB1 inbreastcancercells quantitative expression was observed DNA whichwasSATB byWestern—blotting．3．Single—strandoligos 1-targeting were and thelentivirusvectorwhichcontainedRNAicassette designedsynthesized．Then WasusedtotransductintoMDA-MB一231．Inthelast lentivims part，SATB RNAi 1-targeting inhibitedthe ofSATB1 inMDA--MB-·231 observedReal-·time expression gene