2010 选修 人工胰.pptVIP

The SEM micrographs are of an islets encapsulated alginate-chitosan-PEG （bead fixed with 0.02% GA） SEM： Scanning electron?microscopy  Assessment of mechanical?stability One hundred capsules were placed in 100 ml of 0.9% NaCl solution in 500 ml flasks. The flasks were put in a shaker at 150 rpm at 37°C. All ruptured capsules as a function of time. The experiments were repeated in an unbiased way 3 times, and the ruptured capsules were enumerated for statistical evaluation Membrane permeability?measurements Survival of Microencapsulated Adult Pig Islets  Survival of Microencapsulated Adult Pig Islets Encapsulation was carried out according to the technique developed in our laboratory (23) using a 3.3% wt/vol concentration of highly purified alginate (donated by John Holahan, Pharmacia, Peapack, NJ) with the following properties: endotoxin content 100 endotoxin unit/g alginate; viscosity = 900 cento Pascal; Mannuronic acid (M)/Guluronic acid (G) ratio: 61% M/39% G which is called high M alginate. After overnight culture, islets were suspended in this alginate at a concentration of 5000 islet equivalents (IE)/mL of alginate. The islet-alginate suspension was loaded into tubing connected to a pump and an air-driven droplet generator. Alginate microcapsules were formed by cross-linking with BaCl2 (20 mM) dissolved in sterile distilled water. Microencapsulation of adult pig islets prolonged the survival of islets for up to 170 days and greater than 193 days for islets in SAC or DAC capsules, respectively (Figure 2A and 2B). ?????????????????????????????????????????? The development of alternative vitrification solutionsfor microencapsulated islets Biomaterials , (2008): islets were encapsulated in an agarose hydrogel 琼脂糖 Microencapsulation of islets in agarose hydrogel Islets were isolated from a Syrian hamster (8 weeks, female) by collagenase digestion method [10]. The islets were cultured for 6 days to remove damaged cells and alter their irregular