X-5型树脂分离纯化黑柴胡黄酮及抗氧化性研究资料.doc

X-5型树脂分离纯化黑柴胡黄酮及抗氧化性研究 李 帅 胡继荣 刘军军 何 杰 马兴元 吴冬青* （河西学院化学化工学院 甘肃省高校河西走廊特色资源利用省级重点实验室 甘肃张掖734000） 摘要：目的 以黑柴胡为原料，通过X-5大孔吸附树脂对其黄酮类化合物进行分离纯化，并测定了黑柴胡黄酮体外抗氧化活性。方法 以静态吸附率和解吸率为指标，考察提取液浓度、提取液pH、静态吸附时间、洗脱液浓度等影响因素，对纯化物进行?DPPH、ABTS+清除能力及还原力实验。结果 X-5大孔吸附树脂分离纯化黑柴胡黄酮的最佳工艺为：提取液的浓度0.369mg/mL，提取液pH 1～2，静态吸附时间7h，45%乙醇和65%乙醇洗脱出黄酮含量较高。纯化后的黄酮液对?DPPH、ABTS+清除能力及还原力均高于未纯化黄酮液。结论X-5大孔吸附树脂是分离纯化黑柴胡黄酮较优的树脂。 关键词：黑柴胡；黄酮；分离纯化；抗氧化性中图分类号：　　　　　　　 文献标识码：Ａ The Purification using X-5 Macroporous Resin and Antioxidant Activity of Flavonoids from Bupleurum smithii Wolff LI Shuai HU Ji-rong LIU Jun-jun HE Jie MA Xing-yuan WU Dong-qing* (College of Chemistry Chemical Engineering Hexi University Key Laboratory Hexi Corridor Resources Utilization of Gansu Universities Zhangye Gansu 734000) Abstract: Objective To investigate the optimum conditions of purification for flavonoids in Bupleurum smithii Wolff using X-5 macroporous resin and evaluate antioxidant activity of total flavonoids. Methods In terms of static adsorption and desorption rate, the optimum conditions including pH, time of extracting solution and the content of elution were discussed. And the antioxidant activity of flavonoids purified were evaluated by scavenging ?DPPH、ABTS+ and reducing power. Results The optimal extracting process of the flavonoids are as follows: pH1-2 and the content 0.369mg/mL of extracting solution, 7h of static adsorption time, the 45% or 65% ethanol as the eluent. The antioxidant activity of purification of flavonoids with macroporous resin is better than ones without. Conclusions It is significant for the purification of flavonoids in Bupleurum smithii Wolff by using X-5 macroporous resin. Key words:Bupleurum smithii Wolff; X-5 macroporous resin ;flavonoids; antioxidant activity 黑柴胡（Bupleurum smithii Wolff），别称小五台柴胡、小五吕柴胡、杨家坪柴胡，是伞形科柴胡属多年生草本植物, 其主要分布于河北、山西、陕西、河南、青海、甘肃等省区，味苦、性微寒，具有解热、抗炎等功效[1]。大孔吸附树脂具有吸附快、容量大、洗脱率高、可再生等优点[2]，因而被广泛应用于分离黄酮、多糖、生物碱等化合物[3-5]。本文利用X-5大孔吸附树脂对黑柴胡黄酮进行分离纯化，并对纯化前后黑柴胡黄酮清除·DPPH、ABTS+能力及还原力进行比较，旨在为分离