产黄酮成分的绶草内生真菌的鉴定.pdfVIP

Mycosystema 菌 物 学 报 15 January 2011, 30(1): 133-137 jwxt@ ISSN1672-6472 CN11-5180Q ©2011 Institute of Microbiology, CAS, all rights reserved. 产黄酮成分的绶草内生真菌的鉴定 * 刘紫英 宜春学院化学与生物工程学院 江西 336000 摘 要：利用薄层层析法进行的定性分析表明，分离自绶草 Spiranthes sinensis 的 3 株内生真菌产生了黄酮类化合物，在形 态学鉴定的同时，扩增、测序了 3 株内生真菌的 rDNA 的ITS 序列，利用 MEGA4.0 软件和邻接法（Neighbour-joining methods ） 进行了聚类分析，菌株 S-1 鉴定为不等弯孢 Curvularia inaequalis，S-2 和 S-3 鉴定为嗜松青霉 Penicillium pinophilum 。 关键词：绶草，不等弯孢，嗜松青霉 Endophytic fungi producing flavonoids from Spiranthes sinensis * LIU Zi-Ying College of Chemistry and Bioengineering, Yichun University, Jiangxi 336000, China Abstract: Thin-layer chromatography (TLC) analysis indicated that three strains S-1, S-2, and S-3 of endophytic fungi isolated from roots, stems and leaves of Spiranthes sinensis could produce flavonoid. To identify these 3 strains, ITS DNA regions of 3 strains were amplified through PCR reaction and sequenced. The phylogenetic tree was constructed by the software MEGA (version 4.0) using Neighbour-joining methods. Morphological observation and molecular biology identification indicated that the strain S-1 was Curvularia inaequalis., and S-2 and S-3 were Penicillium pinophilum. Key words: Spiranthes sinensis, Curvularia inaequalis, Penicillium pinophilum 绶草 Spiranthes sinensis (Pers.) Ames 为国家二 （Lin et al. 2000，2001 ；Peng 2008 ）。基于植物内 级濒危保护的植物，根及全草入药，药名盘龙参。 生真菌能够产生与宿主相同或相似化学成分的认 盘龙参中已报道分离出黄酮类化合物spirathetin- Ⅰ、 识（Huang et al. 2008；Schulz et al. 2002），本研究 阿魏酸酯、二氢菲类衍生物和甾醇类等多种化合物 从绶草 S. sinensis 的根、茎和叶中分离、纯化获得 基金项目：江西省教育厅科技计划项目（No. GJJ09606 ） * Corresponding author. E-mail: yingziliu2008@163.com 收稿日期: 2010-09-15, 接受日期: 2010-11-23 134