桑木层孔菌液体培养过程中几种胞外酶活性的变化.pdfVIP

Mycosystema 菌 物 学 报 15 March 2011, 30(2): 275-280 jwxt@ ISSN1672-6472 CN11-5180Q ©2011 Institute of Microbiology, CAS, all rights reserved. 桑木层孔菌液体培养过程中几种胞外酶活性的变化 1 1* 2 曹春蕾 崔宝凯 秦问敏 1 北京林业大学微生物研究所 北京 100083 2 中国科学院沈阳应用生态研究所 沈阳 110016 摘 要：研究了桑木层孔菌 Phellinus mori 液体培养过程中发酵液中淀粉酶、果胶酶、羧甲基纤维素酶（CMC 酶）和漆酶等 4 种胞外酶的活性变化，同时测定了蛋白质、还原糖和 pH 的变化。结果表明，桑木层孔菌拥有丰富的胞外酶系，淀粉酶酶 活第 7 天时达到最大值，果胶酶在第 9 天和第 12 天的酶活都较高，羧甲基纤维素酶第 10 天时酶活最高，漆酶酶活第 9 天时 达到最大值，说明桑木层孔菌对淀粉类物质利用最早。蛋白质浓度在第 8 天和第 11 天时出现两个峰值，还原糖浓度随培养 时间逐渐降低，发酵液pH 值在培养初期逐渐变小，后期逐渐变大。 关键词：淀粉酶，果胶酶，羧甲基纤维素酶，漆酶，酶活 Activity changes of several extracellular enzymes in liquid culture of Phellinus mori 1 1* 2 CAO Chun-Lei CUI Bao-Kai QIN Wen-Min 1Institute of Microbiology, Beijing Forestry University, Beijing 100083, China 2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China Abstract: Phellinus mori had rich extracellular enzymes. The amylase activity in liquid culture of the fungus had the maximum value on day 7. Pectinase showed two peaks on day 9 and 12, respectively. The maximum value of carboxymethyl cellulose enzyme (CMCase) activity appeared on day 10. The laccase reached its peak on day 9. It was indicated that Phellinus mori preferred starch to pectin, cellulose and lignin. The protein concentration had two peaks on day 8 and 11, respectively. The reducing sugar concentration decreased gradually with the incubation time, and the pH value decreased on days 2-7 but increased after day 8. Key words: amylase, pectinase, carboxymethyl cellulose enzyme, laccase, enzyme activity 基金项目：中央高校基本科研业务费专项资金（No. BLYX200912 ）；国家自然科学基金（No. 30910103907 ） * Corresponding author. E-mail: baokaicui@ 收稿日期: 2010-12-07, 接受日期: 2011-01-24 276