bcl-2、bx在乳腺增生组织的表达及温阳散结法治疗乳腺增生病的机理研究.pdfVIP

乳腺癌分别降低；Bax的表达水平随着乳腺增生-癌前病变-乳腺癌病变程度的加重而 降低。动物实验研究显示通过对照组与空白组组织病理形态及 Bcl-2、Bax 的比较， 具有明显差异，说明雌激素、黄体酮联合诱导建立大鼠乳腺增生模型造模成功；应用 温阳散结法治疗大鼠乳腺增生，在组织形态学上可减轻乳腺增生的病变程度，在分子 水平上可下调Bcl-2、上调Bax的表达。 关键词 乳腺增生病；温阳散结法；Bcl-2 ；Bax Bcl-2, Bax expression in mammary gland hyperplasia and temperature study on the mechanism of hyperplasia of mammary glands treated by Yang Jie Speciality：SurgeryofTCM Author ：KaoShugang ， Tutor：Prof.SunYi an Abstract Objective: Clinical observation on hyperplasia of mammary glands of human disease, breast cancer, breast cancer Bcl-2 of apoptosis gene in precancerous lesions and Bax expressions, experimental study on the method of warming Yang Jie on treatment of hyperplasia of mammary glands in rats and combat apoptosis gene (Bcl-2) and apoptosis genes (Bax) effect, promote the study of cell apoptosis mechanism of hyperplasia of mammary glands treated by Chinese herbs. Methods: Clinical study on selected human precancerous lesions of breast hyperplasia and breast cancer, wax blocks of breast cancer specimens, immunohistochemical observation of Bcl-2 and expression of Bax in the pathological type. Experimental study on 100 Vister randomly divided into 5 groups of female rats, medial muscle injections of estradiol benzoate on its hind legs 0.5mg/kg/d, 25 days in a row, and then intramuscular progesterone 4mg/kg/d, 5 days in a row. Modeling successful rat (80) randomly divided into four groups: the control group, Chinese medicines, increased milk Ning group, treated with tamoxifen groups, each group of 20. Preparation of rat mammary gland, methods such as through HE staining, IMMUNOHISTOCHEMISTRY, observation of breast tissue morphology, and cell apoptosis resistant ge