“形寒饮冷则伤”的模拟研究与实质分析.pdfVIP

Simulative study and essential analysis on “eating cold food in cold temperature will hurt the lung” Speciality：Medical Department Of TCM Author ：Zhang Yang Tutor：Prof. Zhang Wei Abstract AIM: To deep discuss Chinese Medicine classical theory “eating cold food in cold temperature will hurt the lung” through simulated atmosphere in the lab, and appraise concerned guidelines’ inspection. Method: Choose 56 healthy Wistar male rats, and divide them into 4 groups randomly, they are: control group, cold-temperature group, cold food-eating group, eating cold food in cold temperature group; 14 ones for each group. Put them into different temperature-controlled boxes or give them different stimulation. During this period, observe their changes, such as their skin, fur, food-eating, stool quality and behaviors. After 7 days, collect samples, observe their pathological changes in pulmonary tissue; exam serum CRP by radioimmunoassay method, the changes of pulmonary tissue homogenate IL-8 contents; exam β alexin’s expression in pulmonary tissue through enzyme immunoassay; exam TGFβ1-mRNA’s expression in pulmonary tissue and bronchus through Situ Hybridization Method. Making statistical treating to the experimental data by applying SPSS13.0 statistics software, and analyze the results. Result: The rats in cold-temperature group, cold food-eating group and eating cold food in cold temperature group, whose pulmonary tissues have obvious changes; i.e. serum CRP rose, IL-8 con