复方健耳剂对da小鼠血清超氧化物歧化酶(sod)和丙二醛(mda)的影响.pdfVIP

目 录 中文摘要 ···············································2 英文摘要 ···············································3 引 言 ·················································5 正 文 ·················································7 1. 实验材料和方法 ······································7 2. 实验结果 ·············································9 3 .讨论 ·················································13 4 .存在的问题展望 ·····································34 5. 结 论〃·············································3 6 参考文献 ··············································37 附 录 ················································4 2 综 述 ················································44 致 谢 ················································56 攻读学位期间发表的学术论文 目录 ···················5 7 作者声明 ··············································58 个人简历 ··············································59 1 中 文 摘 要 目 的：观察中药“复方健耳剂”对 DBA 小鼠血清超氧化物歧化 酶（ ）、丙二醛（ ）的影响，从而为阐述防治老年性耳聋提供依 SOD MDA 据。 方 法：将36 只DBA 小鼠随机分为3 组：A 组为 13 只3 个月DBA 小鼠正常对照组，B 组为 13 只3 个月DBA 小鼠复方健耳剂组，C 组为 10 只6 个月DBA 小鼠复方健耳剂组，B 组为出生断奶后开始以复方健耳 剂为饮用水，服到第 3 个月，C 组为出生断奶后开始以复方健耳剂为饮 用水，服到第6 个月。最后将小鼠麻醉后摘眼球取血约 1.0ml，离心取上 清液，采用分光光度计检测血清的波长，从而测出小鼠血清超氧化物歧 化酶（SOD ）、丙二醛（MDA ）变化。 结 果：B 组DBA 小鼠超氧化物歧化酶（SOD ）高于A 组DBA 小 鼠(P 均小于0.05) ，C 组DBA 小鼠超氧化物歧化酶（SOD ）高于A 组DBA 小鼠(P 均小于0.05) ，B 组和C 组，差异无显著意义（P 均大于0.05 ）。B 组DBA 小鼠丙二醛（MDA ）低于A 组DBA 小鼠(P 均小于0.05) ，C 组 DBA 小鼠丙二醛（MDA ）低于A 组DBA 小鼠(P 均小于0.05) ，B 组和C 组，差异无显著意义（P 均大于0.05 ）。 结 论：中药“复方健耳剂”对DBA 小鼠有保护抗氧化物酶系统 的机制，从而判断其具有清除自由基，保护线粒，对抗细胞凋亡的作用。 关键词： 小鼠，耳聋，复方健耳剂，SOD，MDA 2 The protection and mechanism research to presbycusis of Compound rehabilitation ear agent abstract