安胃汤对大鼠慢萎缩性胃炎egf和egfr表达的pcr研究.pdfVIP

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安胃汤对大鼠慢萎缩性胃炎egf和egfr表达的pcr研究.pdf

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安胃汤对大鼠慢萎缩性胃炎egf和egfr表达的pcr研究

目录中文摘要················································1 英文摘要 ················································3 引言 ·················································7 正文 ·················································9 1 . 实验材料和方法··········································9 1. 1 实验材料···············································9 1.2实验方法·············································〃〃〃〃·11 1.3 统计学方法 ········································1 6 2 . 实验结果··················································16 2.1 动物外观观察··········································· 16 2.2 胃粘膜大体形态观察·····································16 2.3 胃粘膜光学显微镜观察··································· 16 2 . 4 血清中表皮生长因子及其受体含量检测结果············17 2.5 实时荧光定量PCR结果···········································19 2 .6 电泳图片·····················································19 3 讨论·················································20 4 . 存在的问题和展望·······································32 结论 ···············································34 参考文献 ···············································3 5 附录 ···············································3 9 综述 ···············································40 致谢 ···············································4 8 攻读学位期间发表的学术论文目录 ···················4 9 作者声明 ·············································50 个人简历 ···············································51 中文摘要目的: 观察安胃汤对慢性萎缩性胃炎大鼠血中表皮生长因子及其受体表达量的影响，从而探讨安胃汤治疗慢性萎缩性胃炎疗效的分子机制，从基因水平上阐释名老中医治疗慢性萎缩性胃炎的学术理论。方法: 健康清洁级雄性Wistar 大鼠84 只，体重 140~ 170 克，完全随机分为空白对照组（A 组）、病理模型组（B 组）、安胃汤大剂量组（C 组）、安胃汤中剂量组（D 组）、安胃汤小剂量组（E 组）、维酶素对照组（F 组）、胃复春对照组（G 组）共七组，每组 12 只。空白组给予正常饮食进水，其余六组采用甲基硝基亚硝基胍（MNNG ）溶液建立慢性萎缩性胃炎大鼠模型。于造模成功后开始，安胃汤大剂量组、安胃汤中剂量组、安胃汤小剂量组、维酶素对照组、胃复春对照组分别给予药物灌胃治疗4 周；空白组，病理模型组同时给予每天每只 2ml 生理盐水灌胃。各组大鼠禁食 12 ｈ，将大鼠麻醉，股动静脉取血，ACD 抗凝，淋巴细胞分离液分离有核血细胞，提取有核细胞总RNA ，经反转录成cDNA ，设计