原创力文档- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
浅刺调督综合疗对脑梗塞大鼠神经细胞保护作用的实验研究
目 录
中文摘要····················································1
英文摘要 ····················································2
引 言 ·····················································4
正 文 ·····················································5
1.实验材料和方法············································5
1.1 实验材料·················································5
1.1.1 实验动物···············································5
1.1.2 药物与试剂·············································5
1.1.3 主要仪器 ···············································5
1.2 实验方法·················································5
1.2.1 脑梗塞动物模型制作法 ···································5
1.2.2模型判断标准 ···········································6
1.2.3 动物分组 ···············································6
1.2.4 操作方法 ···············································6
1.3 取材与观察指标 ···········································7
1.3.1 脑组织的采集和固定 ·····································7
1.3.2脑组织BDNF的检测方法 ···································8
1.3.3脑组织c-Fos的检测方法 ··································8
1.3.4观察指标 ···············································8
1.4 统计学方法 ···············································9
2.实验结果··················································9
2.1 实验动物数量分析·········································9
2.2 大鼠神经功能评定·········································9
2.3 苏木素-伊红染色(HE-染色) ·······························10
2.4三个时间点各组大鼠脑组织BDNF表达比较····················10
2.5三个时间点各组大鼠脑组织c-Fos表达比较···················11
3.讨 论 ···················································12
3.1祖国医学对脑梗塞的认识 ··································12
3.2现代医学对脑梗塞的认识 ··································15
3.3 关于脑梗塞模型的制作和神经功能的评价 ····················18
3.4 浅刺调督综合疗法的理论及依据 ····························18
3.4.1 浅刺针法的理论和依据 ··································18
3.4.2调督法治脑梗塞的理论及依据 ····························20
3.4.3 辅以补阳还伍汤的理论和依据 ····························21
3.5脑梗塞与脑源性神经生长因子BDNF 的关系···········
文档评论（0）