水稻内生菌的分离纯化与鉴定03较完整解析.docVIP

玉林师范学院本科生毕业论文 Separate and morphologic observation of endophyte in rice 院 系 专 业 生物技术 学 生 班 级 生技0班 姓 名 学 号 20006403136 指导教师单位 指导教师姓名 林谦 指导教师职称 水稻内生菌的分离及形态观察 生技0班 指导教师 林谦 摘要 关键词: 水稻、内生细菌、内生真菌、分离、形态观察 Separate and morphologic observation of endophyte in rice Biotechnology Class 08 Xie Xiang-feng Supervisor Lin-qian Abstract Rice is one of the most important grain crop in the world. It is the primary nutrition source of billionaire population. Along with world population increases every day and arable areas reduce sharply, nowadays, the study of rice increase have became an important topic in agricultural sciences research. Through plating method separates endophyte fungi and bacteria of rice, than adopt enumeration by platecount method to determine or measure the quantity of rice endophytic roots, stems and leaves. Their average value were separate derived. Than we can use F-test to examine whether roots, stems and leaves have significant difference or not. At last, electron microscope observe morphology of this endophyte fungi and bacteria in rice. The bacterial growth tendency of stem, root and leaf in rice indications that endophytic bacteria in rice plants with roots in the distribution of organs account for the greatest number, followed by leaves, stems at least. However, the growth tendency of stem , root and leaf in rice endophytic fungi indications that endophytic fungi in rice plants with roots in the distribution of organs account for the greatest number. But the distribution of endophytic fungi is significantly less than the endophytic bacteria. Separating endophyte , then indentify endophyte by morphologic observation and electron microscope observation. we can make use of endophyte in rice and leap benefits by researching distribution of plant endophyte in rice. Key words: Rice, Endophytic bacteria, Endophytic fungi, Separate, Morphologic observation 目录 1前言 5 1.1植物内生菌的定义 5 1.2植物内生菌的研究进展 5 1.3植物