差异表达研究方法及其在作物耐旱基因筛选中的应用.pdf

2008 21 2 西 南 农 业 学 报 Vol21 No2 Southw est China Journal ofAgricultural Sciences 513 : 1001- 4829( 2008) 02- 0513- 04 1, 2 1 2 1 符明联 , 王敬乔 , 杨清辉 , 李根泽 ( 1. , 650205; 2. , 650201) : ( SSH)cDNA , PCR( DD-PCR ) ( RDA ) ( ESTs) ( SAGE)cDNA ! # $ ( cDNA-AF P)% ( ) * + , - . / 0 1 2 3 4 / 0 * 5 6 7 , 8 9 3 : ; = ?@AB CD * - . EF GHI , J : ; = KL MN AJ O* - . , EP QR; , ESTURV; W XYRV; Z [ \ , EP] ^ RV; T U_ ` * a b - . c , d e f cDNA-AF P I g : ; V; ; ; h ] : S59. 034 : A App lication of differen tial expression analy sis in screen ing drough t toleran ce genes of crop s 1, 2 1 2 1 FU M ing-lian , WANG Jing-qiao , YANG Q ing-hui, IG en-ze ( 1. Econom ic Research Institute, YunnanA cademy of Agricultural Sciences, Yunnan Kunm ing 650205, China; 2. Yunnan AgriculturalUn-i versity, Yunnan Kunm ing 650201, China) A bstract: Severalm ainm ethods of studying differential gene expression of crops, such as suppression subtractive hybridization ( SSH ), cD- NA m icroarray, differential display PCR ( DD-PCR ), representationaldifference analysis ( RDA ), expressed sequence tags ( EST s), serial ana lysis of gene expression ( SAGE) and cDNA amplified fragm ent length polym orphism ( cDNA-AF P) w ere discribed. By contr