咪唑类离子液体在纤维素酶降解纤维素体系中的作用.pdfVIP

第 66 卷 第 1 期 化 工 学 报 Vol.66 No.1 2015 年 1 月 CIESC Journal January 2015 咪唑类离子液体在纤维素酶降解纤维素体系中的作用 范琳，王少君，李坤兰 （大连工业大学轻工与化学工程学院，辽宁大连 116034 ） 摘要：以 1-乙基-3- 甲基咪唑磷酸二乙酯（[Emim]DEP）、1-乙基-3- 甲基咪唑醋酸（[Emim]Ac）为研究对象，考察 两种离子液体（IL ）对酶法降解纤维素过程的影响。采用超声辅助离子液体溶解纤维素，[Emim]DEP 和[Emim]Ac 分别使纤维素的结晶度降为 53.6%和 62.3% 。在纤维素酶降解再生纤维素的过程中，当IL 的加入量小于2.0%时， 对酶解过程起促进作用，其中加入量为 0.5%时，促进作用最强，酶解率分别提高了 11.4%和 17.5%。在最优条件 下（加入 0.5%IL ），测量纤维素酶系中各个酶的酶活，结果表明离子液体对 β-葡萄糖苷酶起到了明显的促进作用， 分别使该酶酶活提高了 120%和 87%。离子液体降低了纤维二糖对葡聚糖外切酶的抑制作用，提高了酶解效率。 关键词：离子液体；纤维素；纤维素酶；溶解；降解 DOI ：10.11949/j.issn.0438-1157 中图分类号：TQ 352.2 文献标志码：A 文章编号：0438—1157 （2015 ）01—0121—05 Function of imidazolium-based ionic liquids in system of enzymatic degradation of cellulose FAN Lin, WANG Shaojun, LI Kunlan (School of Light Industry Chemical Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China) Abstract: Taking 1-ethyl-3-methylimidazolium diethyl phosphate ([Emim]DEP) and 1-ethyl-3-methylimidazolium acetate ([Emim]Ac) as research targets, the influence of the two ionic liquids (IL) on the process of enzymatic degradation of cellulose was investigated. By using ultrasound to assist ionic liquids in dissolving cellulose, [Emim]DEP and [Emim]Ac made the crystallinity of cellulose decrease to 53.6% and 62.3% respectively. In the process of enzymatic degradation of regenerated cellulose, when the dosage of IL was less than 2.0%, IL acted as promoter, and when the dosage of IL was 0.5%, the promoting effect of IL was the strongest. Enzymatic hydrolysis