华北及西北地区岩黄芪根瘤菌的表型及遗传多样性.pdfVIP

第25 生 态 学 报 vol巧 ，No.5 2005 ACTA ECOLOGICA SINICA May2005 华北及西北地区岩黄蔑根瘤菌的表型及遗传多样性 刘 洋，隋新华 ‘，陈文新 (中国农业大学生物学院，农业部农业微生物资源及其应用重点开放实验室，北京 100094) 摘要:选用分离自河北、内蒙古等5省区的岩黄茂根瘤菌3。株及17株已知参比菌株，进行了营养利用、抗生素抗性、耐逆性及 生理生化反应等125项表型性状研究，所得数值分类树状图表明不同宿主及不同地域的岩黄蔑根瘤菌的表型多样性。通过对其 中部分菌株进行16SrDNAPCR-RFI-P及BOX-PCR指纹图谱分析，聚类结果表明供试岩黄蔑根瘤菌具有遗传多样性。 关键词:岩黄茂根瘤菌;多样性;数值分类;16SrDNAPCR-RFI.P;BOX-PCR指纹图谱 文章编号:1000-0933(2005)05-1088-07 中图分类号:Q938,Q939.11 文献标识码:A Phenotypicandgeneticdiversityofrhizobiaisolatedfrom rootmodulesof Hedysarumspp.growninthenorthernandnorthwesternregionsofChina LIUYang,SUIXin-Hua,‘CHENWen-Xin (CollegeolBiologicalSciences.ChinaAgricultureUniversity,KeyLaboratoryof Agro-MicrobialResourceandApplication,MinistryofAgriculture,Beijing100094,China).ActaEcologicaSiniea,2005,25(5):1088 1094. Abstract:ThelegumespecieswithinthegenusHe勿sarumaredeep-rootedannualorperennialherbs，andtheyarewell distributedinthetemperatezones.About40speciesofHedysarumarenativetoChinaandtheyarepotentialfoliagesandare usedasresourcesofherbalmedicine.Theselegumesarepioneersplants，butitsgeographicdistributionmaybedeterminedby theexistenceofproperrhizobia.Aimingatunderstandingthediversityofrhizobiaassociatedwiththem.weisolatedthirty rhizobialstrainsfrom rootnodulesofHedysarum spp.growninthenorthernandnorthwesternregionsofChinaand characterizedtheminthisstudy. ThephenotypiccharacterizationofthestrainsshowedthatmostoftherhizobialstrainsisolatedfromHedysarumspp.had highstressresistance.Forinstance，allofthemgrewfrompH5.0topH11.0.Over800oofthesestrainsgrewwellinYMA mediumat37Candseveralofthemevengrewat4Corwereresistantto60C.Somestrainshaveatolerancetohigh concentrationoferythromycinorstreptomycin(300pg·ml’)andofNaCl(30o，w/v).Theresistanceofthesestrainstothe reverseconditionsmightb