医学人生详解.ppt

酶化学的哲学启示： 顺应自然，无为而无不为 《老子》： 为学日益，为道日损。损之又损，以至于无为。无为而无不为。 圣人欲不欲，不贵难得之货；学不学，复众人之所过；以辅万物自然而不敢为。 Hippokrates：自然自愈力 《黄帝内经》：其知道者，法于阴阳，和于术数，食饮有节，起居有常，不妄作劳，故能形与神俱，而尽终其天年，度百岁乃去。 化学和医学人生 克服医生的“上帝情结” 抑制化学家生产“新物质”的冲动 我们手握巨大能力 旗帜鲜明的反对转基因农业 ≈ 黄金大米饭配绿菜花 咖喱牛肉牛腩饭 （抗癌食品） 鲑鱼籽蒸米饭 （养颜护肤 ） 胡萝卜素含量 发展转基因不如发展合成生物学 Synthetic biology: Reduce biological systems to their component parts Creating new functions from new combinations of component parts Provide new processes for producing chemicals and drugs Could result in the third industrial revolution over the next 50 years Genetically-Modified Organism: High potential threat to the ecological system Thank you! 感谢倾听！ 兰之猗猗，扬扬其香。众草与茂，无人而芳。 幽幽栖谷，馨配君王。不采而佩，于兰何伤。 时人暗蔽，贤者埋藏。以日以年，逍遥四方。 奕奕荠麦，贸贸雪霜。君子之道，闇然日彰。 幽籣操 导泻剂:胡万林 * * HepG2 cells transiently co-transfected with PPRE-luciferase plasmid and a β-galactosidase vector were treated with 0, 0.8, 8 and 80 μM of VO(acac)2 for 24 h and then the luciferase activity was measured The NIT-1 cells were incubated with 10μM of VO(acac)2 or NaVO3 for 24 hours. Then cells were fixed in cold 4% paraformaldehyde, stained with FITC conjugated antibody for PPARγ (green) and Hochst33258 for nuclei (blue), and observed under a confocal microscopy. * NIT-1 cells were pretreated with 10μM VO(acac)2 or NaVO3 for 24 h, then treated with 50 ng/ml of TNF-α and/or IL-6 for 24 h * * * * amelogenin【医】釉基质 Ameloblast cell【医】成釉质细胞 金岳霖-超级光棍 * * 酶不改变一个化学反应的方向、不使反应的热力学平衡发生移动，但酶改变了反应途径，降低了反应发生需要克服的能量障碍，从而增加反应速率 * 酶通过提高反应的速率，引导反应朝着期待的方向进行 例四 调节蛋白质相互作用 BSOV increased PPARg and adiponectin in fat tissue of db/db diabetic mice Ctrl 0.4 mmol/kg/day PPARg Actin Actin Adiponectin 0.04 mmol/kg/day Vanadium activate PPARg * Upper: VO(acac)2 activated PPRE transcription ctrl VO(acac)2 NaVO3 PPARg Merge Left: vanadium induced PPARγ translocation in NIT-1 Vanadium induced Hsp60-PPARg protein interaction IB IB ctrl VO(acac)2 NaVO3 PPARg Hsp60 IP PPARg IB IB ctrl VO(acac)2 NaVO3