砀山酥梨4CL基因家族的全基因组鉴定与分析.pdfVIP

砀山酥梨4CL 基因家族的全基因组鉴定与分析 曹运鹏, 方志，李姝妹, 闫冲冲, 丁庆庆, 程曦, 林毅, 郭宁*, 蔡永萍* 安徽农业大学生命科学学院，合肥，230036 摘要：4-香豆酸：辅酶A 连接酶(4CL)基因是植物调控木质素代谢，以及参与类黄酮和其他次生代谢产物的合成的关键基因之 一，而木质素的合成及聚合在细胞壁沉积导致部分薄壁细胞次生壁加厚形成石细胞。为了更好的了解砀山酥梨中4CL 基因的 种类和数量，本文利用砀山酥梨基因组的氨基酸和cDNA 数据库对4CL 基因家族进行筛选，分析砀山酥梨基因组中4CL 基因的 种类、进化关系、基因的物理定位、以及基因结构和保守基序。结果显示在砀山酥梨基因组中发现并初步确定了29 个4CL 基 因，通过基因的定位分析发现除了4、8、11、12 号染色体上面没有4CL 基因之外，其他的染色体上都存在有4CL 基因；并且 在 9、17 号染色体上还存在着基因簇。通过基因结构和进化树之间的比较，进一步确定了基因结构和进化之间的相互联系。 这些研究结果为砀山酥梨4CL 基因功能的深入分析奠定了基础。 关键词：砀山酥梨；4CL；基因结构；进化 Genome-wide identification and analyses of 4CL gene families in Pyrus bretschneideri Rehd Yunpeng Cao, Zhi Fang ， Shumei Li, Chongchong Yan, QiongQiong Ding， Xi Cheng, Yi Lin, Ning Guo*, Yongping Cai* College of Chemistry and Life Sciences，Anhui Agricultural University ，Hefei 230036 Abstract ： 4-Coumaric acid: coenzyme A ligase (4CL) gene is one of the key genes involved in the regulation of lignin metabolism and the synthesis of flavonoid and other secondary metabolites in plant, while the synthesized and polymerized lignin is deposited in cell walls and leads to thickening of secondary walls in some parenchyma cells and formation of stone cells. To better understand the variety and quantity of 4CL genes in Pyrus bretschneideri Rehd., we used the amino acid and cDNA databases of Pyrus bretschneideri Rehd. genome to screen 4CL gene family, and analyzed their classification, evolutionary relationships, physical location, gene structure and conserved motif. Our results showed that 29 4CL genes were identified and preliminarily characterized, and these 4CL genes were distributed in all chromosomes except chromosomes 4, 8, 11, 12 and clustered on chromosomes 9 and 17 through gene location analysis. The relationship between 4CL gene structure and evolution was further determined by comparing gene structure and phylogenetic tree. These findings provide a basis for further analysis of 4CL gene function in Pyrus bretschneideri Rehd. Key