斯坦福大学分子生物学课件-Replication3A课件.pptVIP

Replication complexes during the cell cycle The Structure of ARS1(S. cerevisiae) * Formation of pre-Replicative complex G2 M ORC MCM MCM CDC6 + Cdt1 Replication Machinery “Pre-Replicative Complex” ORC CDC6+ Cdt1 S late G1 early G1 Eukaryotic initiation complex ORC : A six subunit protein complex which has been implicated as being the eukaryotic DNA replication initiator protein. Subunits are named according to their size, with ORC1 being the largest and ORC6 being the smallest subunit. Yeast ORC specifically binds to replication origins in an ATP dependent manner and has been shown to possess