毛细管气相色谱分析法在中药分析中应用.docVIP

毛细管气相色谱分析法在中药分析中的应用Capillary gas chromatography (CGC) high separation efficiency, analysis speed, sample consumption, especially in the analysis of the traditional Chinese medicine complex mixture for separation degree is high, in the traditional packed column on the separation of the material also can be difficult in capillary column on purpose to separate, convenient and quick. Capillary column chromatography system basically and packed column, including injection system, the examination system, the examination system, the recording system. In recent years, chromatographic column manufacturer develops new commodity GC capillary column, high temperature resistant GC capillary column, low loss high inert GC capillary column, special GC capillary column, commodity in the chromatographic column use most containing 5% of phenyl together in methyl silane chromatographic column. In addition, capillary gc qualitative and quantitative analysis, qualitative analysis: preserving value, detector, chemical method qualitative; Quantitative analysis: normalization; External standard, internal standard quantitative. And in traditional Chinese medicine in the API CGC for the content determination, there are many applications, such as: the brain qualitative identification for drug mint, detailed and accurate results are more process, have very good effect and more great progress. Keywords：Capillary column chromatography (CGC), liquid membrane thin, column capacity small, the peak fast, proprietary Chinese medicine, separating degree is high, commodity GC capillary column, the qualitative and quantitative analysis, API mint content determination of the brain 前言 1956年，Golay开始研究涂壁空心毛细管色谱柱，于同年研制出来第一支玻璃毛细管开管柱[1], 毛细管柱具有柱效高和分析速度快的特点。这种柱的内径只有0. 2-0. 5mm，固定液的厚度0. 3-1. Sum，而柱长达数十至数百米，理论塔板数可达1护因而柱效极高。1957年发表了他的研究成果，并且从理论上分析了影响柱效的因素[2]，这标志着一个全新的毛细管柱(开管柱)气相色谱法的诞生。 近年来毛细血管气相色谱法（CGC）以其分离效率高，分析速度快，样品用量少等特点，在中成药复杂混合物的分析检分离度高，在传统填充柱上难分离的物质可在毛细管色谱柱上达到分离目的。如李永庆等对解毒药康灵片中微量东莨菪碱成功检测，灵敏度且结果准确可靠即为一例。【3】