甲基戊糖梭菌膜整合焦磷酸酶基因的烟草转化及转基因植株的鉴定资料.docVIP

甲基戊糖梭菌膜整合焦磷酸酶基因的烟草转化及转基因植株的鉴定 摘要：本已构建的pET(CmPP)质粒为模板，再扩增CmPP，然后通过酶切克隆到质粒pBI121中，构建植物表达载体pBI121(CmPP)，并将其转化到根癌农杆菌LBA4404中。通过农杆菌侵染的叶盘转化法和多重PCR鉴定，获得了阳性CmPP转基因烟草植株。 关键词：甲基戊糖梭菌膜整合焦磷酸酶农杆菌转基因烟草 Tmembrane-integral pyrophosphatase gene of Clostridium methylpentosum and identification of the transgenic tobacco plants Abstract: In this work, using the constructed plasmid pET(CmPP) as DNA template, the membrane-integral pyrophosphatase gene CmPP of Clostridium methylpentosum was re-amplified, and subcloned into the plasmid pBI121 by enzyme digestion to create the plant expression vector pBI121(CmPP) that