希夫碱Cu教程详解.doc

希夫碱Cu(Ⅱ)配合物的合成及其与DNA的相互作用研究 Synthesis of Schiff base Cu(Ⅱ) complex and its Interaction with DNA  Synthesis of Schiff base Cu(Ⅱ) complex and its Interaction with Deoxyribonucleic acid (DNA) is an essential life material and the carrier of genetic information in organism. It is closely related to normal life activities such as growth, development and propagation and abnormal life activities such as carcinogenesis. Meanwhile, DNA is targeting molecule for many anticancer drugs. Hence it is meaning that interactions of small molecules with DNA are investigated to understand bind of DNA with protein, explore and exploit new nucleic acid probe, understand anticancer mechanism at molecular level, elucidate molecular biological mechanisms of carcinogenesis and teratogenicity, and to provide theoretical guidance for designing effective clinical anticancer drugs. Binding studies of transition metal complexes have become a very important field in the development of DNA molecule probes and chemotherapeutics in recent years. Schiff base complexes become a research hotspot because their specific activities of pharmacology and physiology. In this paper, the progress about o-phenylendiamine creacts with Salicylic aldehyde to form (salicylidene)-o-phenylenediamine (Schiff base),the Schiff base to treat with cupric acetate to form Schiff base Cu(Ⅱ) complex, and its structure had been characterized by IR, elementary analysis. Ct- DNA-binding of platinum complex had been investigated by means of UV spectra, The results have shown that the binding mode of platinum complex to ct-DNA is the intercalative mode. Key word: Salicylic aldehyde，o-phenylendiamine，ct-DNA 目录 1前 言 1 1.1引言 1 1.2 金属配合物与DNA在遗传物质中的研究进展 1 1.2.1 DNA的研究前情 1 1.2.2 Cu的应用前景 2 1.2.3希夫碱过渡金属配合物在生命科学领域的应用 2 1.2.4论文的选题依据和目的 3 2实验部分 4 2.1实验试剂和仪器 4 2.1.1试剂 4 2.1.2仪器 5 2.2二乙胺基水杨甲醛缩邻苯二胺希夫碱过渡金属( Cu) 配合物方法和分析 5 2.2.1邻苯二胺缩二乙胺基水杨醛配体的合成 5 2.2.2邻苯二胺缩二乙胺基水杨醛希夫碱铜（Ⅱ）配位合物的合成 5 2.3溶液的配制 6 2.4紫外实验方案 6 2.4.1配合物在缓冲