Atomic Abs.-1s要点.pptVIP

sample flow in the (HGAAS) 1. The metal oxyanions reacts with Sodium Borohydride and HCl and produces a volatile hydride: H2Te, H2Se, H3As, H3Sb, etc. 2. The peristaltic pump and the flowing reagents along with the tubing of specific lengths controls the time from reagent mixing and separation of the volatile hydride from the liquid and sending the sample to the optical cell. 3. The liquid mixture flows into a gas/liquid separator where the hydride and some gaseous hydrogen are purged (via a high purity inert gas) into the optical cell via a gas transfer line 4. In the optical cell the flame is ignited automatically by the air/C2 H 4 and the gaseous metal hydride form decomposes into the elemental form which can absorb the HCLs beam. The light passes to the Mon. and then to the PMT Gas liquid separator Optical cell peristaltic pump Mixing reaction coil Volatile Hydride AAS Interferences Any factor that affects the ground state population of the analyte element. Factors that may affect the ability of the instrument to read this parameter. A) Spectral interferences: due to radiation overlapping than of the light source. B) Formation of compounds that do not dissociate in the flame. The most common example is the formation of calcium and strontium phosphates. C) Ionization of the analyte reduces the signal. This is commonly happens to barium, calcium, strontium, sodium and potassium. D) Matrix interferences: due to differences between surface tension and viscosity of test solutions and standards . E) Broadening of a spectral line 1. Doppler effect 2. Lorentz effect 3. Quenching effect 4. Self absorption or self-reversal effect * K L M N O P ? Atoms:Incase of atoms only electronic transition occurs. Molecular absorption spectra Atomic absorption spectra The outer most electrons occupy ?, ? or n electronic energy in the ground state. 1- The outer most electrons occupy one of the atomic orbitals and have its energy levels [K, L, M