纸品中痕量荧光物质的萃取检测详解.docVIP

纸品中痕量荧光物质的检测 摘 要 荧光增白剂常添加于纸浆中用于纸品的增白，其中二苯乙烯类荧光增白剂应用最为广泛。荧光增白剂易被人体吸入，加重肝脏负担；进入伤口中，会与蛋白质反应，从而影响伤口愈合速度；若长期大量接触荧光增白剂，会成为潜在致癌因素。现行荧光增白剂的定量方法少，操作复杂，没有统一规范的标准方法。通过以以VBL作为标准物质，测定不同浓度下的荧光强度以建立标准曲线，对比不同pH条件下常温提取、不同温度加热提取、不同时间超声提取和微波提取的效果，最终确立以微波处理120s的方式提取纸样中荧光增白剂的方法来检测各种生活用纸中荧光增白剂的定量检测方法。该标准物质发射波长和激发波长分别为435nm、335nm;在0.01μg/ml-2.00μg/ml范围内建立定量检测标准曲线为y（a.u）=45.53681x-0.60702，线性相关性R2=0.99982；平均加标回收率为94.577％，满足定量检测需求。通过对18种不同实际样进行荧光增白剂含量的测定，对部分纸样中二苯乙烯类荧光增白剂-VBL的含量有了一定了解。所采用的方法灵敏，操作简便，检测限低，为荧光增白剂的定量检测提供了一种可靠准确的方法。 关键词：荧光增白剂—VBl，微波消解，超声处理，生活用纸 Purification and detection of trace fluorescent substances in paper products ABSTRACT Fluorescent whitening agent is added to bleaching of pulp used in paper products, with stilbene type fluorescent whitening agent most widely used. Fluorescent brightener is easy to be inhaled, and add to the burden of liver; enter the wound will react with proteins, thus affecting the wound to heal ;if long-term exposure of fluorescent whitening agent, could be a potential cancer-causing factors. Fewer existing quantitative method of fluorescent whitening agent, operating complex, no standardization standard methods.By using VBL as the standard substance, to establish the standard curve of the fluorescence intensity was measured at different concentrations, different pH under the condition of normal temperature extraction, different temperature, different extracting time of ultrasonic extraction and microwave extraction effect, finally established 120s in microwave method extraction method of fluorescent whitening agent pattern to detect fluorescent brightener agent method for quantitative detection of various living paper. The emission wavelength and excitation wavelength of the standard material is 435nm and 335nm respectively; in the range from 0.01μg/ml to 2.00μg/ml establish quantitative detection standard curve for y(a.u.) = 45.53681 x - 0.60702 ,with the linear correlation R squared = 0.99982 and the average spiking rate of 94.577%, which meet the