- 1、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。。
- 2、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 4、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 5、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
自测题(第二单元)静定梁、刚架内力计算
答案
一、1 X 2 X 3 X 4 O 5 X 6 X
二、1 A 2 B 3 C 4 C
三、
1、 2 外侧
2、 0 , 0 (各 2.5 分 )
3、 CB , CD (或 ACD ) (4 分)
4、 -8kN ( 5 分 )
5、 -30kN ( 5 分 )
四、
(5 分)
五、
= 0 ,=P , =3P ( 2 分)
图 每 杆 (3 分), 图 名( 1 分)
图
六、
=3 kN , =6 kN , =3 kN ( 2 分)
图 每 杆(2.4 分), 图 名 单 位(1 分)
图 ( kN·m )
七、
=12 kN ,可 不 求 竖 向 反 力 ;
= 4 kN ; =1 kN ( 2 分)
图 每 杆( 2.4 分), 图 名,单 位( 1 分 )
图 ( kN·m )
八、
( 15 分 )
九、
每杆 (2.5分)
十、
( 15 分 )
十一、
Q 图 (kN)(2 分) N 图 (kN)(2 分) (5 分)
Acknowledgments
The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supported
by a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the National
Science Council, which were awarded to Y.C. Song (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC
103-2321-B-400-008).
Author Contributions
Y.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. and
S.J.L. wrote the manuscript.
Additional Information
C57BL/6 mice were immunized subcutaneously (s.c.)
once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were
harvested, and the response of IFN-γ -secreting cells was determined by ELISPOT after 48 h of peptide
stimulation. Briefly, 2 × 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptide
in an anti-IFN-γ -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells were
removed, and a biotinylated anti-IFN-γ Ab (eBioscience, San Diego, CA, USA) was added to each well.
The plates were incubated at 37 °C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience,
CA, USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, MO,
USA) staining solution. The reaction was stopped after 4–6 min by placing the plate under tap water.
The spots were counted using a
文档评论(0)