Pb胁迫下小桐子体内的6种元素含量研究 毕业论文.doc

Pb胁迫下小桐子体内的6种元素含量研究 Study on six kinds of elements contents in Jatropha Curcas under Lead stress 摘 要 本试验采用不同浓度的Pb2+浇施小桐子幼苗，利用火焰原子吸收分光光度法对小桐子各部位中的Mn、Ca、Mg、Cu、Pb 、Zn 六种元素的含量进行测定，研究Pb胁迫下小桐子各部位对6种元素的吸收特点及各元素之间的关系，结果表明小桐子经Pb胁迫后：根、茎、叶、叶柄四个部位对Pb的吸收量都随着Pb胁迫浓度的升高而增大，各部位在同等浓度条件下对Pb吸收量存在明显差异，其吸收量表现为叶片＞根＞叶柄＞茎；Cu在对照组植株体内各部位的含量分布关系为叶片＞根＞叶柄茎，而处理组分布关系表现为根＞叶柄＞叶片＞茎；处理组与对照组相比，各部位对Ca、Mg、Mn的吸收都有所增加。试验证明小桐子能够累积Cu离子、Pb离子，对Pb有一定的耐受性，同时还证明了Pb的胁迫能提高小桐子对Ca、Mg、Mn的吸收。 关键词：小桐子；火焰原子吸收分光光度法；Pb2+胁迫；吸收特点 ABSTRACT In Pb2+stress,the study researched the reLationship Jatropha Curcas and six elements (Mn,Mg,Ca,Cu,Pb and Zn) and the traits of six elements absorption with FAAS method determining the content of six elements in Jatropha Curcas bodies.The results showed that after Jatropha stress by Pb:roots, stems,Leaves and petiole of four parts of the absorption of Pb with the Pb stress concentrations are increasing,and the site conditions at the same concentration of apparent absorption of Pb differences,the absorption performance of Leafrootpetiolestem;Cu in the control group plants of various parts of the relationship of content of leafrootpetiolestem,the distribution between the treatment groups showed rootpetioleleafstem;rootpetioleleafstem ;treatment group compared with the control group,each part of the Ca,Mg,Mn absorption has increased.The experiment reveaLed that not only Jatropha Curcas couLd accuLumate ions of Copper and PLumbum, it had some tolerance,but aLso demonstrated Pb couLd improve the absorption of calcium,Mg and Mn. Key words: Jatropha Curcas;The fLame atom absorbs and divides all Luminosity Law; Pb2+ stress; Absorb the characteristic 目 录 1.前言 1 2.材料与方法 2 2.1材料 2 2.2实验仪器 2 2.2.1主要试剂 2 2.3培养方法 2 2.3.1培养液及培养基质 2 2.3.2种子处理及萌发实验 2 2.3.3幼苗的前期培养 3 2.3.4 Pb金属胁迫培养 3 2.4测定方法 3 2.4.1仪器工作条件参数 3 2.4.2样品溶液制备 3 2.4.3校准曲线绘制 3 2.4.4样品测定 4 3.结果与分析 4 3.1 Pb胁迫下Pb在小桐子各部位的含量分布 5 3.2 Pb胁迫下Cu在小桐子各部位的含量分布 5 3.3 Pb胁迫下Ca在小桐子各部位的含量分布 5 3.4 Pb胁迫下Mn在小桐子各部位的含量分布 6 3.5 Pb胁迫下Mg在小桐子各部位的含量分布 6 3.6 Pb胁迫下