长距离实时定量PCR技术及在DNA损伤的研究中的应用.pdf

生物技术通报 ·技术与方法· BIOTECHNOLOGY BULLETIN 年第 期 2013 5 长距离实时定量 PCR 技术及其在 DNA 损伤 研究中的应用 1 1 2 1 梁姣  王淑红  张子平  王艺磊 （1. 集美大学水产学院，厦门 361021 ；2. 西东大学生物系，新泽西州 07079） 摘 要 : DNA 损伤作为评价环境化合物的遗传毒性、生物个体环境暴露水平和肿瘤罹患危险度的指标越来越受到人们的关 注。检测DNA 损伤的方法很多，而PCR 技术由于所需样品量少，检测灵敏度高等优点，近年来发展很快。综述PCR 技术在DNA 损伤研究中的应用，并重点介绍新近发展的长距离实时定量PCR （long amplicon real-time quantitative PCR ，LA-real time QPCR ）的 关键技术。 关键词 : 长距离实时定量PCR DNA 损伤 环境化合物 - Long Amplicon Real time Quantitative PCR Technology and Its Application in DNA Damage Detection 1 1 2 1 Liang Jiao Wang Shuhong Zhang Ziping Wang Yilei （1. ， ， 361021 ；2. ， ， Fisheries College Jimei University Xiamen Department of Biological Science Seton Hall University New Jersey 07079 ） Abstract:  DNA damage as an endpoint for evaluating genotoxicity of environmental compounds and organism levels of environmental exposure as well as the risk of tumor is always the research focus. Several methods were developed for detecting DNA damage, PCR technology, due to its little DNA needs and high accuracy is widely used in DNA damage detection recently. Here, we review the application of PCR on DNA damage detection, more over, the technical key steps of a newly developed technology called the long amplicon real-time quantitative PCR （LA-real t