激发子PebC1原核表达蛋白的生物活性分析.pdf

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN 2011年第12期 激发子 PebC1 原核表达蛋白的生物活性分析 张云华 杨秀芬 曾洪梅 袁京京 邱德文 （中国农业科学院植物保护研究所 农业部生物防治重点开放实验室，北京 100081） 摘 要: 将激发子 PebC1 基因在大肠杆菌中进行表达，重组蛋白可以明显促进植物生长，诱导植物产生抗旱性和抗病性。用 不同浓度的重组表达蛋白 PebC1 处理番茄，其幼苗的株高分别增加了 24.56%-48.34% ；同时诱导了番茄对灰霉病的系统抗性，番 茄的病叶率和病情指数均明显下降，诱抗效果达到 34.06%-52.44%。小麦经重组表达蛋白 PebC1 处理后，叶片的抗衰度和幼苗存 活率也明显增加，抗旱综合系数从 15.28 提高到了 64.88。 关键词: 激发子 重组表达蛋白 生物活性 Biological Activity Analysis of a Novel Protein Elicitor PebC1 Expressed in Prokaryote Zhang Yunhua Yang Xiufen Zeng Hongmei Yuan Jingjing Qiu Dewen （Key Laboratory for Biological Control of Ministry of Agriculture ， Institute of Plant Protection ， Chinese Academy of Agricultural Sciences ， Beijing 100081 ） Abstract: The gene of PebC1， a novel protein elicitor， was expressed in E.coli ， and recombinant protein would obviously promote the growth of plants， inducing the plant resisting the drought and the disease. Treating the tomato by different concentrations of the recombinant protein PebC1， the height of seedlings increased 24.56%-48.34%， respectively. In the same time， the recombination protein PebC1 also - induced tomato disease resistance to gray mold. The rate of tomatos ill leaves and disease index declined apparently， the effect of which could be 34.06%-52.44%. Seedling height and its length of descending axis were also obvious high after the wheat treated by the recombination protein PebC1. Coefficient of comprehensive wheat drought tolerance also enhanced from 15.28 to 64.88. Key words: Elicitor The recombinant pro