葡萄乙醇脱氢酶基因Ⅲ的电子克隆及其生物信息学分析.pdf

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2009 5 武雪 黄晓丽 王喆之 ( , 7 10062) : 利用电子克隆方法获得葡萄乙醇脱氢酶基因 (ADH ) , 并采用生物 息学方法对该基因编码蛋白从氨基酸 组成理化性质跨膜结构域疏水性/亲水性亚细胞定位高级结构以及功能域等方面进行了预测和分析结果表明, 葡萄 ADH 基因全长 1 602 bp, 包含 1 140 bp 的 ORF, 编码 379 个氨基酸, 该蛋白不具有明显的疏水区域, 也无跨膜结构域, 螺旋 和不规则卷曲是其二级结构的主要构件葡萄 ADH包含有 ADH 功能域, 和其他植物的 ADH在序列组成高级结构及活 性位点等方面均具有高度的相似性 : 乙醇脱氢酶 电子克隆 生物 息学 Electronic Cloning and Characterizations ofADH Gene from Vitis vinfi era Using Bioinformatics Tools W u X ue H uang X iao i W ang Zhezh i (K ey Laboratory of M inistry of Educationf orM edicinalP lant Resources and N aturalPharmaceutical Chem istry, College of Lfi e ciences, hanx iN ormal University, X ian 710062) A bstrac:t A n a coho dehydrogenase (ADH ) gene from Vitis vinfiera w as c oned by e ectronic c oning based on the EST se quences from U nigene of NCBI Som e characters of theADH that can encode am ino ac id w ere ana y zed and pred icted by the too s of b io info rm atics in the fo ow ing aspec ts, inc uding the composition o f am ino acid sequences, hydrophobic ity o r hydrophi ic ity, secondary and tertia ry struc ture of prote in and functionR esu ts show ed that the fu eng th of ADH from Vv inif era w as 1 602 bp ong and it conta ined a com p ete ORF w h ich encoded 379 am ino acidThe encoded pro tein had no t obv iou s hydrophob ic ity reg ion and transm em brane structure w as not conta inedT he m a in m o tif o f pred icted secondary structu re o f ADH w ere a pha he ix and random co iT he sequence, advanced structure and ac tiv ity sites o f ADH that conta ined an ADH doma in w ere high y conserv