塞来昔布联合替吉奥对胃癌裸鼠移植瘤生长及其淋巴管生成的影响.pdfVIP

Med J Chin PLA, Vol. 38, No. 12, December 1, 2013 　967 论　著 塞来昔布联合替吉奥对胃癌裸鼠移植瘤生长及淋巴管生成 的影响 孟翠翠，罗治彬 [摘要]　目的　探讨选择性环氧化酶2抑制剂塞来昔布联合替吉奥对人胃癌裸鼠皮下移植瘤生长及淋巴管生成的 影响及其可能机制。方法　建立胃癌裸鼠皮下移植瘤模型，成瘤后将裸鼠随机分为4组(n=6) ：阴性对照组、塞来昔布 组、替吉奥组、塞来昔布联合替吉奥组(联合给药组) 。连续给药2 1d后，取材，测瘤重，计算抑瘤率及给药前后裸鼠体 重变化，评价药物治疗的毒副作用。应用免疫组化方法检测各组肿瘤组织中COX-2 、VEGF-C蛋白表达情况并计数淋巴 管密度。结果　药物治疗组裸鼠并未出现明显的不良反应，体重均较治疗前明显增加(P 0.05) 。塞来昔布组、替吉奥 组、联合给药组的抑瘤率分别为32.7 1% 、4 8. 13% 、79.44% 。塞来昔布组、联合给药组COX-2 、VEGF-C表达及淋巴管密 度明显低于对照组、替吉奥组(P 0.05) ，替吉奥组COX-2 、VEGF-C表达及淋巴管密度与对照组比较差异无统计学意义 (P 0.05) 。结论　塞来昔布、替吉奥单独作用均可明显抑制肿瘤生长，且二者应用具有协同抑瘤作用。塞来昔布单用 或联合替吉奥均可通过下调COX-2表达、进而下调VEGF-C 的表达而抑制肿瘤组织淋巴管生成。 [关键词]　塞来昔布；替吉奥；环氧化酶2抑制剂；血管内皮生长因子C ；淋巴管生成 [中图分类号]　R73　　　　[文献标志码]　A　　　　[文章编号]　0577-7402 (20 13) 12-0967-05 [DOI]　10. 11855/j.issn.0577-7402.20 13. 12.03 Effect of celecoxib combined with tegafur, gimeracil and oteracil potassium on growth and lymphangiogenesis of the xenograft tumor of gastric cancer in nude mice 1 2 MENG Cui-cui , LUO Zhi-bin 1Department of Radiotherapy, Changzhou Cancer Hospital of Soochow University, Changzhou, Jiangsu 213001, China 2Department of Oncology, Third Peoples Hospital of Chongqing, Chongqing 400016, China * Corresponding author, E-mail: luozhibincq@126.com [Abstract]　Objective　To inv estigate th e effect and analy ze th e p ossibl e mechanism of cel ecox ib combined w ith tegafur, gimeracil and oteracil p otassium on grow th and ly mphangiogenesis of sub cutaneously transplanted human gastric cancer in nude mi ce. Methods　Human gastri c cancer w as transpl anted sub cutaneously su ccessfully in nu de mi ce. W h en th e l argest diam eter of tumor reach ed ab out 5mm, th e nu de mice w ere randomly div ided into f our group s (6 each): control group, cel ecox ib group, tegafur+gimeracil +oteracil p otassium group, and celecox ib