海藻糖及在生物工程方面的应用.pdfVIP

生物技术通报 BI OTECHN OLOG Y B ULLETI N 2005 6 * 1 1 ** 2 ** 1 陈杰 何聪芬 叶兴国 董银卯 1 2 ( , 100037; , 100081) : 自海藻糖发现以来对其化学性质生理功能作用 理代谢途径等已进行了较为深入的研究, 其分 子生物学的研究也渐渐兴起研究表明海藻糖能提高生物体对干旱低温高温pH 盐渍等逆境条件下的抗性 离体试验表明海藻糖能保护生物膜蛋白质的结构并能保持逆境下的酶活性, 同时, 外源海藻糖同样对生物体有保 护作用由于海藻糖具有这些独特的生物学功能, 它已在许多方面得以应用, 可作为食品工业的一种添加剂和甜 味剂,使干燥食品在得水后保持原有的色香味; 也可作为医药工业的非特异性生物制品和生化药品保护剂,使其 在常温下保存, 从而降低运输与储存费用; 另外, 在农业研究中可利用现代分子生物技术培育表达海藻糖的转基因 作物, 提高农作物的抗旱抗冻等抗逆性能 : 海藻糖 抗逆 生物学功能 合成途径 转基因 Trehalose and Its Application in Bioengineering 1 1 2 1 Chen Jie He Congfen Ye Xingguo Dong Yinmao ( 1 Beij ing Te chnology an d Business Univ ersity , K ey lab of P lan t Resourc es R esearc h and D ev elop ment , Beij ing 100037; 2 nst itute of Cr op S cience s, Chinese A cade my of A g ricult ural S cience s, Beij ing 100081) Abstract: Since the iscovery of trehalos, it has been eeply stu ie on chemical property , physiological func- tion, w orking mechanism an metabolic pathw ay. Its molecular biological reseaches are also gra ually emphasize . St u ies have show n that the en ogenous t rehalose can raise the resistance of organism against rought, low tempera- ture, high temperature, unstability pH , salt an so on. Experiment in vitro in icate that trehalose can protect the membrane, protein st ruct ure an maint ain the activities of some enzymes on st ress con ition. Moreover, exogenesis trehalose also can protect the organism from the amage of abiotic stress. Because of those particular characters, trehalose has been applie in many fiel s inclu