羟基红花黄色素a对油酸-脂多糖所致大鼠急性肺损伤的保护作用.pdfVIP

940 药学学报 Acta Pharmaceutica Sinica 2010, 45 (7) : 940944 羟基红花黄色素A 对油酸-脂多糖所致大鼠急性肺损伤的保护作用 王晓菲, 金 鸣, 童 静, 吴 伟, 李金荣, 臧宝霞* (首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所药理研究室, 北京 100029) 关键词: 羟基红花黄色素A; 急性肺损伤; 肺水肿; 炎症因子; 油酸; 脂多糖 中图分类号: R285 文献标识码:A 文章编号: 05 13-4870 (2010) 07-0940-05 Protective effect of hydroxysafflor yellow A against acute lung injury induced by oleic acid and lipopolysaccharide in rats * WANG Xiao-fei, JIN Ming, TONG Jing, WU Wei, LI Jin-rong, ZANG Bao-xia (Depa rtment of Pharmacology, Beij ing Anzhen Hosp ital, Capital Medical University -Beij ing Institute of Heart Lung and Blood Vessel Diseases, Beij ing 100029, China) Abstract: This study is to investigate the pharmacological effect and mechanism of action of hydroxysafflor yellow A (HSYA) on acute lung injury (ALI). The rat ALI was induced by oleic acid and lipopolysaccharide (LPS) inj ection . The incidence of acidosis, PaO2 (arterial blood oxygen pressure), W/D (wet weight/dry weight) and lung index (LI) were measured. Electron microscope and optical microscope were applied to observe lung morphological changes in rat. RT-PCR was used to determine TNF- and ICAM-1 mRNA level. Inhibition effect of HSYA on plasma inflammatory cytokine expression was measured by ELISA. HSYA could alleviate pulmonary edema, reduce acidosis, keep PaO2 from descending, inhibit inflammatory cell infiltration, inhibit rat lung TNF- and ICAM- 1 mRNA expression and plasma IL-6 and IL-1β level elevation. HSYA is an effective ingredient to remit ALI induced by oleic acid and LPS in rat. Key words: hydroxysafflor yellow A; acute lung