内源性胱硫脒-γ-裂解酶硫化氢调控hepg2细胞凋亡.pdfVIP

下载本文档

6
0
约4.28万字
约 8页
2016-05-14 发布于安徽
举报
版权申诉

内源性胱硫脒-γ-裂解酶硫化氢调控hepg2细胞凋亡.pdf

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

药学学报 Acta Pharmaceutica Sinica 20 13, 48 (8): 1233 1240 ·1233 · Endogenous cystathionine- -lyase/hydrogen sulfide pathway regulates apoptosis of HepG2 cells * WANG Tian-xiao , SHI Xiao-yan, LIU Ying-hua (Ins titute of Chinese Materia Medica, College of Pharmacy, Henan University, Kaifeng 4 75004, China ) Abstract : This study is to investigate the role of endogenous CSE/H 2 S in regulating apoptosis of HepG2 cells. MTT and Trypan blue assay were performed to determine the effect of CSE inhibitor PAG and CSE siRNA on proliferation of HepG2. Production of H S from HepG2 cells was assessed spectrophotometrically 2 using N , N -dimethyl-p -phenylenediamine-dihydrochloride. Cells apoptosis was detected by means of double staining of Hoechst 33342 and PI with Array Scan V TIHCS600 High-Contents. Dihydroethidine (DHE) and 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) assay was used to determine intracellular superoxide anion and ROS level. Reduced glutathione (GSH) was determined by OxiSelect ™ Total Glutathione Assay Kit. Recombinant plasmid pcDNA 3.1/myc-His( )-CSE was constructed and transfected into 293T cells to rescue the ROS and GSH level to further investigate the effect of CSE/H S on ROS and GSH. Western blotting was 2 performed to test the effect of CSE siRNA on expression of activated caspase 3 and p-AKT and Nrf2 protein. The results showe