基因组和分子遗传育种 Rice Genomics.pptVIP

Location of the pms1(t) locus on the molecular linkage map of chromosome 7 To calculate the linkage distance between molecular markers and pms1(t) locus, the data were analyzed with MAPMAKER/EXP 3.0 (Lincoln et al., 1992) at a limit of detection (LOD) threshold of 3.0. A linkage map was constructed using MapDraw V2.01 (Liu and Meng, 2003). Primer sequences and sizes of polymorphic bands for the SSR and In-Del markers in the pms1(t) region High-resolution genetic and physical map of the pms1(t) locus In the partial genetic map of the molecular markers, the numbers under the long horizontal line indicate the times of recombination events detected between the pms1(t) locus and its corresponding markers. The bold bars represent the BAC clones. The pms1(t) locus was covered by the thick horizontal line between the two vertical dashed lines from RM21242 to YF11, in which the genomic information was based on Nipponbare genome sequence (released 4.0), and some gene annotations of the gene loci represented with black bars were from TIGR database Gene annotations of 14 gene loci from RM21242 to YF11 on chromosome 7 Candidate genes of pms1(t) Among these loci, LOC_Os07g12130 encodes a Myb-like protein containing a DNA-binding domain, which hits 16 gene ontology (GO) terms. One of the gene ontology (GO) terms is GO:0009628, which responds to abiotic stimulus. Interestingly, GO:0009266, a child term for GO:0009628, responds to thermal stimulus. Two other second child terms, GO:0009408 and GO:0040040, also respond to heat stimulus and have the characteristics of thermosensory behavior, which are very closely related to the thermo-sensitive genic male sterile characteristics. However, for the other 13 candidate genes, we did not find a direct relationship with the photoperiod or thermo-sensitive response. These results suggest that the locus of LOC_Os07g12130 is more likely a thermo-sensitive candidate gene for pms1(t). Semi-quantitative RT-PCR results of five candidate gene