精浆游离MicroRNA 34―b在特发性少精子症及无精子症中的表达和临床应用.docVIP

精浆游离MicroRNA 34―b在特发性少精子症及无精子症中的表达和临床应用 　　[摘要] 目的 研究游离MicroRNA 34-b（miR-34b）在特发性少精子症和特发性无精子症患者精浆中的表达及诊断价值。 方法 收集特发性少精子症、特发性无精子症和同期因女方因素不孕前来检查的生育力评估正常的健康男性精液各20份，采用实时荧光定量PCR检测精浆游离miR-34b的相对含量，通过方差分析统计各组间的差异，并对精浆游离miR-34b在特发性少精子症和特发性无精子症中的诊断价值进行ROC曲线分析。 结果 正常对照组、特发性少精子症组和特发性无精子症组三组间精浆miR-34b表达量差异有高度统计学意义（F=21.44，P中国论文网 /6/view-7217518.htm 　　[关键词] 特发性少精子症；特发性无精子症；miR-34b；精浆 　　[中图分类号] R698.2 [文献标识码] A [文章编号] 1673-9701（2015）25-0004-04 　　The expression and clinical role of seminal plasma free MicroRNA 34-b in patients with idiopathic oligospermia and azoospermia 　　LOU Jiangtao WEI Renxiong YU Liangliang SHI Bo CUI Yun 　　Male Division Laboratory， Ningbo Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medicine University， Ningbo 315012， China 　　[Abstract] Objective To quantify the expression of seminal plasma free miR-34b in patients with idiopathic oligospermia and azoospermia and to analyze the diagnostic value of it. Methods 20 cases semen of Idiopathic oligospermia and azoospermia were collected， respectively. At the same time， 20 cases semen of healthy male with fertility evaluation which because of the woman factor lead to infertility were collected as control group. the relative expression of seminal plasma free miR-34b was detected by real-time fluorescent quantitative PCR， the dates were analyzed by variance analysis， the diagnostic value of seminal plasma free miR-34b in diseases of oligospermia and azoospermia were analyzed by ROC curve. Results The relative expression of seminal plasma free miR-34b in oligospermia group and azoospermia group were decreased compared with normal controls（P 　　1 材料与方法 　　1.1 病例选择 　　选取2012年8月～2014年11月来我院男科就诊的特发性少精子症20例，年龄分别为25～39岁，平均（32.1±4.4）岁和特发性无精子症患者20例，年龄26～42岁，平均（31.5±4.5）岁。精液常规分析3次以上且均为少精或3000 r/min离心15 min后显微镜观察均为无精，每次精液检测间隔2个月，排除精液量异常、血精、液化不良、畸形精子症、精液白细胞数≥1×106/mL以及高热病史、自身免疫性疾病和接触化学及放射性物质的相关职业者。另外选择同期因女方因素造成不孕，生育能力评估正常的健康男性20例为正常对照组，年龄25～39岁，平均（29.6±3.4）岁。各组在年龄结构、婚龄等基线资料方面经齐同性检验差异无统计学意义（P0.05），具有可