尾巨桉再生体系的建立—-李桂英.docVIP

尾桉再生体系的建立摘要：MS + IAA 0.05 mg/L + LC 2.0 mg/L + Vc 100 mg/L + 蔗糖 30g/L + 琼脂 7g/L（pH 5.8-6.0）；（2）MS + NAA0.05 mg/L + 6-BA 1.0 mg/L + 腐胺500μmol/L + 亚精胺 100μmol/L + Vc 100mg/L + 蔗糖 30 g/L + 琼脂 7 g/L（pH 5.8- 6.0）；（3）1/2 MS + NAA 0.05 mg/L+ LC 0.8 mg/L + Vc 100 mg/L +蔗糖30 g/L + 琼脂 7 g/L（pH 5.8-6.0）；（4）1/2MS + IBA 0.5 mg/L + Vc 100 mg/L+ 蔗糖30 g/L + 琼脂 7 g/L（pH5.8-6.0Establishment of the Regeneration System for Eucalyptus grandis ×Eucalyptus urophylla Li Guiying Life Science and Technology School, Zhanjiang Normal University, Zhanjiang 524048 Abstract: Using the stem segments of Eucalyptus urophylla Sterile clones as explants, the regeneration system from callus to regeneration plants was established by conducting tissue culture experiments and obtaining callus with differentiation potential, differentiation of adventitious buds, adventitious shoot elongation and rooting.The optimum culture conditions for the Eucalyptus urophylla clone regeneration system were obtained by comparing the variety of combinations of different growth regulators: (1)Callus induction culture media: MS + IAA 0.05 mg/L + LC 2.0 mg/L + Vc 100 mg/L + Sucrose 30 g/L + Agar 7 g/L（pH5.8-6.0）; (2)Adventitious buds induction culture media： + NAA 0.05 mg/L +6-BA 1.0 mg/L + Putrescine 500μmol/L + Spermidine 100μmol/L + Vc 100mg/L + Socrose 30g /L + Agar 7 g/L（pH5.8- 6.0）; (3)Adventitious buds elongation culture media：1/2MS+ NAA 0.05 mg/L + LC 0.8 mg/L + Vc 100 mg/L + Socrose 30 g /L + Agar 7 g/L（pH5.8-6.0）; (4)Rooting culture media：1/2MS + IBA 0.5 mg/L + Vc 100 mg/L+ Socrose 30 g/L + Agar 7 g/L（pH5.8-6.0Key words: Eucalyptus grandis ×Eucalyptus urophylla; Regeneration system; Tissue culture 桉树( Eucalyptus) ,桃金娘科(Myrtle family)，桉属（Eucalyptus） 广泛栽培于广东、广西、福建、云南和海南等省区[]。 桉属树种是异花授粉的多年生木本植物，杂种后代严重分离变异，用有性繁殖方法很难保持优良树种的特性，采用激素虽能解决其扦插，压条、驳枝等繁殖方法的生根问题，但大量繁殖仍有一定困难[2]。为此，国内外均已开展桉树组织研究，其中大约已有30种桉树从其各种器官组织诱导出愈伤组织，然而，桉树作为含酚类丰富的木本植物，从愈伤组织获得再生植株有相当大的难度，目前成功获得再生植株的只有10多个种[3]。 建立桉树组织培养与再生系统 ,既可为商品化大规模育苗做准备 ,又有助于建立遗传转化体系 ,为利用