PGC-1α蛋白对结肠癌细胞侵袭和转移的影响及机制研究技术分析.docxVIP

PGC-1α蛋白对结肠癌细胞侵袭和转移的影响及机制研究 摘要：目的 研究PGC-1α蛋白对结肠癌细胞侵袭和转移的影响及机制。方法 收集我院2012年6月至2015年3月期间我院接受手术治疗的结肠癌患者组织样本及临床病理资料，分析PGC-1α蛋白的表达与结肠癌临床病理参数的相关性。划痕实验及Transwell法检测PGC-1α蛋白沉默后对细胞侵袭及转移的影响。Western blot法检测PGC-1α蛋白对侵袭相关蛋白MMP-2及MMP-9表达的影响。结果 PGC-1αα蛋白的表达与结肠癌的肿瘤大小、淋巴结转移、及远处转移密切相关（P＜0.05），与患者性别、年龄没有相关性(P＞0.05)。沉默PGC-1α蛋白可以明显抑制结肠癌HCT116细胞的侵袭及转移能力，同时下调MMP-2及MMP-9的表达。结论 PGC-1αα蛋白可以通过影响MMP-2及MMP-9的表达影响结肠癌细胞的侵袭转移。 关键词：PGC-1α蛋白；结肠癌；侵袭；转移 Effect of PGC-1α on invasion and metastasis of colon cancer cells and its mechanism Objective： To study the effect and mechanism of PGC-1αon colon cancer cells invasion and metastasis.Method：To collect the tissue samples and clinical data of patients with colon cancer treated by surgery in our hospital from March 2015 to June 2012, and analyze the correlation between PGC-1αexpression and clinical pathological parameters. The effect of PGC-1αon cell invasion and metastasis was application of scratch test and Transwell method. Used Westernblot to detect the effect of the expression of MMP-2 and MMP-9 associated with cells invasion after silent PGC-1αexpression.Results: the expression of PGC-1α was closely related to tumor size, lymph node metastasis and distant metastasis (P 0.05), and there was no correlation between sex and age of patients (P 0.05). Silencing of PGC-1α can significantly inhibit the invasion and metastasis of colon cancer HCT116 cells, and Down the expression of MMP-9 and MMP-2.Conclusion: PGC-1α can affect the invasion and metastasis of colon cancer cells by affecting the expression of MMP-9 and MMP-2. Key words: PGC-1α; colon cancer; invasion; metastasis 前言 PGC-1α属于PGC-1家族的一员，在机体的能量代谢中发挥着重要的作用。其作为过氧化物酶体增殖物激活受体γ（peroxisome proliferator-activated receptor γ，PPARγ）的辅激活因子 ADDIN KyMedRef2008REF:REF [1]，可以影响心脏病，肥胖，糖尿病等机体的代谢过程 ADDIN KyMedRef2008REF:REF [2-4]。同时在肿瘤细胞的研究中也发现PGC-1α可以调节多种肿瘤细胞包括肝癌，结肠癌，乳腺癌等多种肿瘤的细胞代谢，进而影响细胞的耐药，增殖等生物学行为 ADDIN KyMedRef2008REF:REF [5, 6]。新的研究发现PGC-1α的表达可能与肿瘤的侵袭及转移能力密切相关 ADDIN KyMedRef2008REF:REF [