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生物信息学 实验报告 班级： 姓名： 学号： 日期： 实验一 核酸和蛋白质序列数据的使用 实验目的 了解常用的序列数据库，掌握基本的序列数据信息的查询方法。 教学基本要求 了解和熟悉NCBI 核酸和蛋白质序列数据库，可以使用BLAST进行序列搜索，解读BLAST 搜索结果，可以利用PHI-BLAST 等工具进行蛋白质序列的结构域搜索，解读蛋白质序列信息，可以在蛋白质三维数据库中查询相关结构信息并进行显示。 实验内容提要 在序列数据库中查找某条基因序列（BRCA1），通过相关一系列数据库的搜索、比对与结果解释，回答以下问题： 1. 该基因的基本功能？ 2. 编码的蛋白质序列是怎样的？ 3. 该蛋白质有没有保守的功能结构域 (NCBI CD-search)？ 4. 该蛋白质的功能是怎样的？ 5. 该蛋白质的三级结构是什么？如果没有的话，和它最相似的同源物的结 构是什么样子的？给出示意图。 实验结果及结论 1. 该基因的基本功能？ This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009] ?2. 编码的蛋白质序列是怎样的？ [Homo sapiens] 1 mdlsalrvee vqnvinamqk ilecpiclel ikepvstkcd hifckfcmlk llnqkkgpsq 61 cplcknditk rslqestrfs qlveellkii cafqldtgle yansynfakk ennspehlkd 121 evsiiqsmgy rnrakrllqs epenpslqet slsvqlsnlg tvrtlrtkqr iqpqktsvyi 181 elgsdssedt vnkatycsvg dqellqitpq gtrdeislds akkaacefse tdvtntehhq 241 psnndlntte kraa