木瓜蓝莓基因组学书籍库简介.pptVIP

Construction and content BBGD is a relational database built on SQLServer2000 and is housed at the Beltsville Agricultural Research Center in Beltsville, MD, USA. The database was implemented on a server running Windows 2000 Server and Internet Information Server (IIS 5.0). The web interface uses active server pages (ASP) and ASP.Net scripts, written in visual basic, to query the backend database. BBGD is divided into two separate but related entities, a sequence database and a microarray database. This allows for the correlation of gene function, deduced from the EST data, with expression levels, deduced from the microarray data. The bulk of the EST and microarray data held at the BBGD currently deals with identifying cold-responsive genes in blueberry flower buds. Utility and discussion The BBGD web site acts as a gateway to the microarray and EST sequencing projects that have been implemented to identify cold-responsive genes in blueberry (fig. 1). In addition, it provides a wealth of general information on blueberry for the public. Figure 1. A snap shot of BBGDs main web page that shows the wealth of information that is available through the web site and the general capabilities of the database. Description BBGD is the worlds first database for blueberry genomics. BBGD is both a sequence and gene expression database. It stores both EST and microarray data and allows scientists to correlate expression profiles with gene function. BBGD is a public online database. Presently, the main focus of the database is the identification of genes in blueberry that are significantly induced or suppressed after low temperature exposure. Conclusion By using the database, researchers have developed EST-based markers for mapping and have identified a number of candidate cold tolerance genes that are highly expressed in blueberry flower buds after exposure to low temperatures. BBGD is the worlds first database for blueberry genomics. BBGD is both a sequence and gene expression