灵芝粗多糖对小鼠胚胎成纤维细胞的抗氧化生物活性研究.docVIP

安徽大学江淮学院 本科毕业论文（设计） 题　　目：霍山灵芝粗多糖对小鼠胚胎成纤维细胞的抗氧 化生物活性研究 学生姓名：　　　刘巍　　 学号： 系 别： 　 理工部 　 专业：　生物信息技术 入学时间：　 　 2012　　　 年　　　 9 　　　月 导师姓名：　　 吴庆喜　　 职称/学位：讲师/博士  霍山灵芝粗多糖对小鼠胚胎成纤维细胞的抗氧化生物活性研究 摘要 目的：对小鼠胚胎成纤维细胞3T3细胞）抗氧化生物活性影响，证明霍山野生灵芝多糖可以保护细胞氧化损伤。方法：相对存活率，接着用MTT测定不同浓度的野生灵芝多糖t-BHP诱导作用下3T3细胞的保护作用。结果： t-BHP的细胞存活率t-BHP浓度为200 μg/ml时，细胞存活率降至75.56%，并且随着t-BHP呈负相关t-BHP浓度为800μg/ml时，细胞存活率低至38.09%。t-BHP诱导组相比，当灵芝多糖浓度为200μg/ml，粗多糖处理组的细胞存活率从56.41%提升至61.98%，而且随着多糖浓度的增加呈正相关。结论：可以的存活率，Antioxidant activity studies of crude polysaccharides from Huoshan wild Ganoderma lucidum on the embryonic fibroblasts of mouse Abstract Objective: To explore the antioxidant activity of Huoshan wild Ganoderma lucidum polysaccharides on the embryonic fibroblasts (3T3 cells) of mouse, to prove Huoshan wild Ganoderma lucidum polysaccharides can protect the cells from oxidative damage. Methods: The 3T3 cells were used as the model, first the relative survival rates of t-butyl hydroperoxide (t-BHP) induced were measured by MTT assay with different concentrations, and then measured for different concentrations of wild Ganoderma lucidum polysaccharides on the MTT assay t-BHP under the protective effect induced 3T3 cells. Results: The results showed that t-BHP induced cell viability group was relatively low, and as t-BHP was negatively correlated with increasing concentration. And t-BHP induced group compared with cell viability Huoshan wild fungus polysaccharide treatment group was higher, and with the concentration’s increase of the polysaccharide it was positively correlated. Conclusion: According to the results, Huoshan wild Ganoderma lucidum polysaccharides could improve the survival rate of the cells to a certain extent, protecting the cells from oxidative damage. Keywords：Huoshan wild fungus；Anti-oxidation；Polysaccharides  目录 1.前言 5 1.1灵芝的研究现状 5 1.2灵芝多糖的研究现状 5 1.2.1灵芝多糖分离纯化的研究现状 5 1.2.2灵芝多糖的生物活性研究现状 6 1.3细胞氧化损伤和细胞抗氧化研究进展 7 1.3.1细胞氧化损伤的危害 7 1.3.2细胞抗氧化