ImprovingthenutritionalvalueofGoldenRicethroughincreasedpro摘要.ppt

* Improving the nutritional value of Golden Rice through increased pro-vitamin A content Vitamin A Certain carotenoids, most importantly β-carotene cleave are referred as pro-vitamin A ※a major problem in parts of the developing world; ※permanent blindless; ※incidence and severity of infectious diseases deficiency provide in rice could be a simple and effective complement ubiquitous and self-sustaining farming Golden Rice a characteristic yellow color produce carotenoids in the endospern of the grain genetically modified rice a maximum level of 1.6μɡ/ɡ carotenoids Is that a desired solution for vitamin A deficiency? the limited production of pro-vitamin A the major hurdle the main problem we have to solve a limiting step and major regulatory step the first barriers to carotenoid biosynthesis in wild-type rice endosperm are phytoene synthase and carotene desaturase no phytoene was accumulated, the desaturation of phytoene to lycopene is proceeding efficiently using the crtI gene may still be the limiting factor in this transgenic tissue daffodil PSY protein level daffodil petal Golden Rice endosperm Why provided from daffodil 转入了含有玉米八氢番茄红素合成酶基因质粒载体的玉米愈伤组织 玉米泛素蛋白启动子 空载体对照组 两种质粒的基因带 携带了psy的质粒。有七个相似的质粒，分别携带了七种不同植株（At, Dc, Np, Zm, Ca, Os, Le）的psy 显色类胡萝卜素的总含量 高类胡萝卜素含量的愈伤组织比例 此处所说的高含量是指超过对照组含量的五倍 Psy转基因来源 a high carotenoid content a high proportion of highly colored calli T-DNA used to create Golden Rice as a reference the highest carotenoid content the amounts of carotenoid produced by different psy transgenes varied a number of events were progressed to the next generation excluding rice and daffodil psy the carotenogenic ability was stable and heritable for all psy cDNAs and high levels of carotenoids were observed in seed from homozygous progeny containing the maize psy/crtI tansgenes PSY CRTI Altering the source of the psy transgene has a major impact on the callus carotenoid content as well as that of transgenic rice